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Planta Med 2002; 68(8): 757-759
DOI: 10.1055/s-2002-33808
Letter
© Georg Thieme Verlag Stuttgart · New York

Variation in Content of Taxol and Related Taxanes in Eastern Himalayan Populations of Taxus wallichiana

Swapna Mukherjee1 , Biswajit Ghosh2 , Timir. B. Jha3 , Sumita Jha1
  • 1Centre of Advanced Study, Department of Botany, University of Calcutta, Calcutta, India
  • 2RKMVC College, Rahara, W.B., India
  • 3Darjeeling Govt. College, Darjeeling, W.B., India
Further Information

Publication History

Received: October 23, 2001

Accepted: March 17, 2002

Publication Date:
09 September 2002 (online)

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Abstract

Extraction and analysis of paclitaxel and other taxanes in bark, needle leaves and stem segments of male and female plants of Taxus wallichiana, representing several populations, indicate that significant variation in taxane content exists within the population. Bark accumulated maximum amount of paclitaxel in almost all plants. Populations located at higher altitude tended to accumulate more paclitaxel than lower altitude plants. Seasons in which samples were collected and plant age have also been shown to affect paclitaxel accumulation. No effects of plant sex on paclitaxel content of the plants analyzed were observed. Significant differences in baccatin-III and 10-deacetylbaccatin III content were found to exist in the trees analyzed in this study.

References

  • 1 Arbuck S G, Blaylock A. Taxol: Clinical results and current issues in development. In: Suffness M, editor Taxol®: Science and Application. CRC Press LLC USA; 1995: p. 380-415
    Google Scholar
  • 2 Schiff P B, Fant J, Horwitz S B. Promotion of microtubule assembly in vitro by taxol.  Nature. 1979;  22 665
    PubMedGoogle Scholar
  • 3 Wheeler N C, Jech K, Masters S S, Borbst S W, Alvardo A B, Hoover A J, Snader K M. Effects of genetic, epigenetic and environmental factors on paclitaxel content in Taxus brevifolia and related species.  Journal of Natural Product. 1992;  55 432-40
    CrossrefPubMedGoogle Scholar
  • 4 Wani M C, Taylor M l, Wall M E, Coggon P, McPhail A T. Plant antitumor agents, VI. The isolation and structure of paclitaxel, a novel antileukemic and antitumor agent from Taxus brevifolia .  Journal of American Chemical Society. 1971;  93 2325-27
    PubMedGoogle Scholar
  • 5 Miller R W, Powell R G, Smith C R, Jr. Arnold E, Clardy J. Antileukemic alkaloids from Taxus wallichiana Zucc.  Journal of Organic Chemistry. 1981;  46 1469-71
    CrossrefPubMedGoogle Scholar
  • 6 Georg G I, Gollapudi S R, Gunn C W, Himes R H, Rao B K, Liang X Z, Mirhom Y W, Mitscher L Aa, Vander V D, Ye Q M. A reinvestigation of paclitaxel content of Himalayan Taxus wallichiana Zucc. and a revision of the structure of brevifoliol.  Bioorganic Medical Chemistry. 1993;  3 1345-46
    PubMedGoogle Scholar
  • 7 Vesela D, Saman D, Valterovaa I,Vanek T. Seasonal variations in the content of taxanes in the bark of T. baccata .  Phytochemical Analysis. 1999;  10 319-21
    CrossrefPubMedGoogle Scholar
  • 8 Ingrid H, Poupat C, Ahond A, Guenard D, Gueritte F, Adeline M T, PingWang X, Dempsey D, Brewillet B, Potier P. Seasonal variation of neutral and basic taxoid contents in shoots of Euoropean Yew (T. baccata).  Phytochemistry. 1999;  52 1041-45
    CrossrefPubMedGoogle Scholar
  • 9 Jha S, Sanyal D, Ghosh B, Jha T B. Improved taxol yield in cell suspension cultures of Taxus wallichiana (Himalayan Yew).  Planta Medica. 1998;  64 270-72
    PubMedGoogle Scholar
  • 10 Falzone C A, Benesi A B, Lecomte J TJ. Characterization of paclitaxel in methylene chloride by NMR spectroscopy.  Tetrahedron Letters. 1992;  33 1169-72
    CrossrefPubMedGoogle Scholar

Sumita Jha

Centre of Advanced Study

Department of Botany

University of Calcutta

35, Ballygunge Circular Road

Calcutta 700 019

India

Email: sumita.jha@vsnl.com

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