Issue 25, 2019

Transcription immunoassay: light-up RNA aptamer-based immunoassay using in vitro transcription

Abstract

Here, we present an ultra-enhanced immunoassay for sensitive and reliable biomarker detection using layer-by-layer assembly and transcription-assisted light-up aptamer generation to induce signal amplification. This dendrimer structure-based transcription immunoassay is ∼1500 times more sensitive than commercial fluorescence ELISA, achieving a detection limit of 108 aM.

Graphical abstract: Transcription immunoassay: light-up RNA aptamer-based immunoassay using in vitro transcription

Supplementary files

Article information

Article type
Communication
Submitted
21 Jan 2019
Accepted
27 Feb 2019
First published
28 Feb 2019

Chem. Commun., 2019,55, 3618-3621

Transcription immunoassay: light-up RNA aptamer-based immunoassay using in vitro transcription

J. Sim, J. Byun and Y. Shin, Chem. Commun., 2019, 55, 3618 DOI: 10.1039/C9CC00514E

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