Programmable Mg2+-dependent DNAzyme switch by the catalytic hairpin DNA assembly for dual-signal amplification toward homogeneous analysis of protein and DNA

Shufeng Liu; Chuanbin Cheng; Hongwei Gong; Li Wang

doi:10.1039/C5CC01649E

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Programmable Mg²⁺-dependent DNAzyme switch by the catalytic hairpin DNA assembly for dual-signal amplification toward homogeneous analysis of protein and DNA†

Shufeng Liu,*^a Chuanbin Cheng,^a Hongwei Gong^a and Li Wang^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China
E-mail: sliu@qust.edu.cn

Abstract

The catalytic hairpin DNA assembly-programmed active Mg²⁺-dependent DNAzyme was proposed for dual-signal amplified detection toward protein and DNA. The protein detection was implemented with the further combination of an additional terminal protection strategy. The detection limit toward avidin and target DNA could be achieved as 2 pM and 0.5 pM, respectively, with a high selectivity.

Download options Please wait...

Supplementary files

Supplementary information PDF (741K)

Article information

DOI: https://doi.org/10.1039/C5CC01649E
Article type: Communication
Submitted: 24 Feb 2015
Accepted: 20 Mar 2015
First published: 20 Mar 2015

Download Citation

Chem. Commun., 2015,51, 7364-7367

Permissions

Request permissions

Programmable Mg²⁺-dependent DNAzyme switch by the catalytic hairpin DNA assembly for dual-signal amplification toward homogeneous analysis of protein and DNA

S. Liu, C. Cheng, H. Gong and L. Wang, Chem. Commun., 2015, 51, 7364 DOI: 10.1039/C5CC01649E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications