Issue 19, 2013
From the journal:

Analyst

Dependence of the direct electron transfer activity and adsorption kinetics of cytochrome c on interfacial charge properties

Gui-Xia Wang,ab   Min Wang,a   Zeng-Qiang Wu,a   Wen-Jing Bao,a   Yue Zhoua  and  Xing-Hua Xia*a  

* Corresponding authors

a State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
E-mail: xhxia@nju.edu.cn
Fax: +86 25 83685947
Tel: +86 25 83597436

b College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, China

Abstract

With the advantages of in situ analysis and high surface sensitivity, surface-enhanced infrared absorption spectroscopy in attenuated total reflection mode (ATR-SEIRAS) combined with electrochemical methods has been employed to examine the interfacial direct electron transfer activity and adsorption kinetics of cytochrome c (cyt c). This work presents data on cyt c adsorption onto negatively charged mercaptohexanoic acid (MHA) and positively charged 6-amino-1-hexanethiol (MHN) self-assembled monolayers (SAMs) on gold nanofilm surfaces. The adsorbed cyt c displays a higher apparent electron transfer rate constant (33.5 ± 2.4 s−1) and apparent binding rate constant (73.1 ± 5.2 M−1 s−1) at the MHA SAMs surface than those on the MHN SAMs surface. The results demonstrate that the surface charge density determines the protein adsorption kinetics, while the surface charge character determines the conformation and orientation of proteins assembled which in turn affects the direct electron transfer activity.

Graphical abstract: Dependence of the direct electron transfer activity and adsorption kinetics of cytochrome c on interfacial charge properties

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Article information

DOI
https://doi.org/10.1039/C3AN01042B
Article type
Paper
Submitted
24 May 2013
Accepted
12 Jul 2013
First published
16 Jul 2013

Analyst, 2013,138, 5777-5782

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Dependence of the direct electron transfer activity and adsorption kinetics of cytochrome c on interfacial charge properties

G. Wang, M. Wang, Z. Wu, W. Bao, Y. Zhou and X. Xia, Analyst, 2013, 138, 5777 DOI: 10.1039/C3AN01042B

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