Issue 12, 2012
From the journal:

Nanoscale

Fractal gold modified electrode for ultrasensitive thrombin detection†

Li-Ping Xu,‡*a   Shuqi Wang,‡a   Haifeng Dong,a   Guodong Liu,c   Yongqiang Wen,a   Shutao Wangb  and  Xueji Zhang*a  

* Corresponding authors

a Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China
E-mail: xuliping@ustb.edu.cn, zhangxueji@ustb.edu.cn
Fax: +86-10-82375840
Tel: +86-10-82375840

b Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Laboratory of New Materials, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China

c Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58105, USA

Abstract

We report a label-free and ultrasensitive aptasensor based on a fractal gold modified (FracAu) electrode for thrombin detection with a femtomolar detection limit. The FracAu electrode was prepared by electrodeposition of hydrogen tetrachloroaurate (HAuCl4) onto a bare indium tin oxide (ITO) electrode surface. After this process the electrode was characterized by SEM. A thiol-modified aptamer against thrombin was immobilized on the FracAu electrode through a self-assembling process. Upon thrombin binding, the interfacial electron transfer of the FracAu electrode was perturbed by the formation of an aptamer–thrombin complex. The concentration of thrombin in the sample solution was determined by measuring the change in the oxidation peak current of hydroxymethyl ferrocene (C11H12FeO) with differential pulse voltammetry (DPV). The current response (reduced peak current) had a linear relationship with the logarithm of thrombin concentrations in the range of 10−15 to 10−10 M with a detection limit of 5.7 fM. Furthermore, the as-prepared FracAu electrode exhibited high selectivity. The application of FracAu electrodes may be extended to prepare other types of biosensors, such as immunosensors, enzyme biosensors and DNA biosensors. These results show that FracAu electrodes have great promise for clinical diagnosis of disease-related biomarkers.

Graphical abstract: Fractal gold modified electrode for ultrasensitive thrombin detection

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C2NR30826F
Article type
Paper
Submitted
06 Apr 2012
Accepted
03 May 2012
First published
08 May 2012

Nanoscale, 2012,4, 3786-3790

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Fractal gold modified electrode for ultrasensitive thrombin detection

L. Xu, S. Wang, H. Dong, G. Liu, Y. Wen, S. Wang and X. Zhang, Nanoscale, 2012, 4, 3786 DOI: 10.1039/C2NR30826F

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