Issue 33, 2011
From the journal:

Chemical Communications

Bioluminescent stem-loop probes for highly sensitive nucleic acid detection†

Eric A. Hunta  and  Sapna K. Deo*a  

* Corresponding authors

a Department of Biochemistry & Molecular Biology, University of Miami Miller School of Medicine, Miami, USA
E-mail: sdeo@med.miami.edu
Tel: 305-2434421

Abstract

Here, we report the first bioluminescent stem-loop probe, which is 50 times more sensitive and able to achieve a LOD 25 times lower than fluorescent stem-loop probes. Chemical generation of a signal from Renillaluciferase reduces background noise for improved quantitative utility in nucleic acid biomarker detection.

Graphical abstract: Bioluminescent stem-loop probes for highly sensitive nucleic acid detection

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC13495G
Article type
Communication
Submitted
13 Jun 2011
Accepted
27 Jun 2011
First published
19 Jul 2011

Chem. Commun., 2011,47, 9393-9395

Permissions

Request permissions

Bioluminescent stem-loop probes for highly sensitive nucleic acid detection

E. A. Hunt and S. K. Deo, Chem. Commun., 2011, 47, 9393 DOI: 10.1039/C1CC13495G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements