Issue 3, 2012
From the journal:

Analyst

Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels†

Meng Zhang,a   Shenguang Ge,a   Weiping Li,a   Mei Yan,a   Xianrang Song,b   Jinghua Yu,*a   Wei Xua  and  Jiadong Huanga  

* Corresponding authors

a Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan, China
E-mail: ujn.yujh@gmail.com
Fax: +86-531-82971177

b Cancer Research Center, Shandong Tumor Hospital, Jinan, Shandong Province, PR China

Abstract

In this work, we reported a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor for carcinoembryonic antigen (CEA) on a gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE). The Ru-silica (Ru(bpy)32+-doped silica) capped nanoporous gold (NPG) (Ru-silica@NPG) composite was used as an excellent label with amplification techniques. The NPG was prepared with a simple dealloying strategy, by which silver was dissolved from silver/gold alloys in nitric acid. The primary antibody was immobilized on the AuNPs modified electrode through L-cysteine and glutaraldehyde, and then the antigen and the functionalized Ru-silica@NPG composite labeled secondary antibody were conjugated successively to form a sandwich-type immunocomplex through the specific interaction. The concentrations of CEA were obtained in the range from 1 pg mL−1 to 10 ng mL−1 with a detection limit of 0.8 pg mL−1. The as-proposed ECL immunosensor has the advantages of high sensitivity, specificity and stability and could become a promising technique for tumor marker detection.

Graphical abstract: Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels

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Article information

DOI
https://doi.org/10.1039/C1AN15942A
Article type
Paper
Submitted
09 Oct 2011
Accepted
15 Nov 2011
First published
07 Dec 2011

Analyst, 2012,137, 680-685

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Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels

M. Zhang, S. Ge, W. Li, M. Yan, X. Song, J. Yu, W. Xu and J. Huang, Analyst, 2012, 137, 680 DOI: 10.1039/C1AN15942A

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