Issue 4, 2012
From the journal:

Analyst

Magnetic retrieval of chitosan: Extraction of bioactive constituents from green tea beverage samples

Hong-Fei Zhangab  and  Yan-Ping Shi*a  

* Corresponding authors

a Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, P. R. China
E-mail: shiyp@licp.cas.cn
Fax: +86-931-4968094
Tel: + 86-931-4968208

b Graduate University of Chinese Academy of Sciences, Beijing, P. R. China

Abstract

A new solid-phase extraction mode for magnetic retrieval of chitosan combined with high-performance liquid chromatography-diode array detection was proposed for the pre-concentration and determination of flavonoids in green tea beverage samples. In the experiment, chitosan was used as sorbents for the extraction of target analytes; after completion of the extraction process, Fe3O4 nanoparticles acted as carrier to retrieve chitosan from the sample solution. Some important parameters influenced extraction efficiency of flavonoids, including the extraction mode, amounts of chitosan, pH of sample solution, extraction time, salt addition, amounts of Fe3O4 nanoparticles, desorption solvent and desroption time, were optimized. Under the optimum conditions, the recoveries of analytes done on samples spiked with the target analytes were between 96.4% and 108.6%; relative standard deviations ranged from 0.6% to 8.7%. The correlation coefficients varied from 0.9917 to 0.9988. The limits of detection ranged from 5.4 to 16.8 ng mL−1 at a signal-to-noise ratio of 3. All four different brands of green tea beverage samples were successfully analyzed by the proposed method.

Graphical abstract: Magnetic retrieval of chitosan: Extraction of bioactive constituents from green tea beverage samples

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Article information

DOI
https://doi.org/10.1039/C1AN15873B
Article type
Paper
Submitted
19 Sep 2011
Accepted
11 Nov 2011
First published
13 Dec 2011

Analyst, 2012,137, 910-916

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Magnetic retrieval of chitosan: Extraction of bioactive constituents from green tea beverage samples

H. Zhang and Y. Shi, Analyst, 2012, 137, 910 DOI: 10.1039/C1AN15873B

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