Issue 8, 2010
From the journal:

Organic & Biomolecular Chemistry

Comparative studies on lectin–carbohydrate interactions in low and high density homo- and heteroglycoclusters†

Marta Gómez-García,a   Juan M. Benito,b   Ricardo Gutiérrez-Gallego,c   Alfredo Maestre,d   Carmen Ortiz Mellet,a   José M. García Fernándezb  and  José L. Jiménez Blanco*a  

* Corresponding authors

a Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Apartado 1203, E-41012 Sevilla, Spain
E-mail: jljb@us.es
Fax: +34 954624960
Tel: +34 954559998

b Instituto de Investigaciones Químicas, CSIC, Américo Vespucio 49, Isla de la Cartuja, E-41092 Sevilla, Spain

c IMIM Hospital del MAR & Universidad Pompeu Fabra. Barcelona Biomedical Research Park (PRBB), C/Doctor Aiguader 88, 08003 Barcelona, Spain

d Departamento de Química Física, Facultad de Química, Universidad de Sevilla, Apartado 1203, E-41012 Sevilla, Spain

Abstract

A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of “non-active” structural motifs in the presence of a certain density of “active” glycoligands.

Graphical abstract: Comparative studies on lectin–carbohydrate interactions in low and high density homo- and heteroglycoclusters

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Article information

DOI
https://doi.org/10.1039/B920048G
Article type
Paper
Submitted
25 Sep 2009
Accepted
26 Jan 2010
First published
24 Feb 2010

Org. Biomol. Chem., 2010,8, 1849-1860

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Comparative studies on lectin–carbohydrate interactions in low and high density homo- and heteroglycoclusters

M. Gómez-García, J. M. Benito, R. Gutiérrez-Gallego, A. Maestre, C. O. Mellet, J. M. G. Fernández and J. L. J. Blanco, Org. Biomol. Chem., 2010, 8, 1849 DOI: 10.1039/B920048G

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