Issue 11, 2009
From the journal:

Lab on a Chip

Simple and rapid process for single cell micro-patterning†

Ammar Azioune,a   Marko Storch,a   Michel Bornens,a   Manuel Théryb  and  Matthieu Piel*a  

* Corresponding authors

a Systems Biology for Cell Division and Cell Polarity, Institut Curie, CNRS, UMR144, 26 rue d′Ulm, Paris, France
E-mail: matthieu.piel@curie.fr
Fax: +33 1 5624 6319
Tel: +33 1 5624 6383

b CEA, DSV, DRDC, Laboratoire Biopuces, 17 rue des Martyrs, Grenoble, France

Abstract

We present a simple and environmentally friendly process for cell patterning on glass covered with an ultrathin layer of poly-L-lysine-grafted-polyethylene glycol (PLL-g-PEG) by exposure to deep UV light. The patterned substrates are stable for months in the lab atmosphere before incubation with proteins. Incubation with proteins resulted in well defined patterns, with high feature resolution. RPE-1 cells seeded on fibronectin/fibrinogen–Alexa 488 patterns were constrained for days on the deep UV exposed regions. Finally, large glass plates were patterned with high homogeneity enabling the assembly of micro-patterned microplates in 96-well format.

Graphical abstract: Simple and rapid process for single cell micro-patterning

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Article information

DOI
https://doi.org/10.1039/B821581M
Article type
Technical Note
Submitted
01 Dec 2008
Accepted
18 Feb 2009
First published
10 Mar 2009

Lab Chip, 2009,9, 1640-1642

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Simple and rapid process for single cell micro-patterning

A. Azioune, M. Storch, M. Bornens, M. Théry and M. Piel, Lab Chip, 2009, 9, 1640 DOI: 10.1039/B821581M

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