Issue 1, 2009
From the journal:

Analyst

Improvement of the electrochemical detection of catechol by the use of a carbon nanotube based biosensor

B. Pérez Lópezab  and  A. Merkoçi*abc  

* Corresponding authors

a Nanobioelectronics & Biosensors Group, Institut Català de Nanotecnologia, E-08193 Bellaterra, Barcelona, Catalonia, Spain

b Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain
E-mail: arben.merkoci.icn@uab.es
Fax: +34 935868020
Tel: +34 935868014

c ICREA, E-08193 Bellaterra, Barcelona, Catalonia, Spain

Abstract

Tyrosinase (Tyr) has been used frequently for the detection of phenolic compounds. The development of a biosensor based on this enzyme-integrated carbon nanotube (CNT) epoxy composite electrode (CNTECE) is described in order to perform measurements of catechol. The enzyme is immobilized into a matrix prepared by dispersion of multi-wall CNT (MWCNT) inside the epoxy resin forming a CNT epoxy-biocomposite (CNTEC-Tyr). The use of CNT improves the electronic transference between the enzyme and the electrode surface. The modified electrode was characterized electrochemically by amperometric and voltammetric techniques. An applied potential of −200 mV vs.Ag/AgC1 applied to the biocomposite based electrode was found to be optimal for electrochemical reduction of the enzymatic reaction products (quinones). The biosensor modified with MWCNT is also compared with a tyrosinase biosensor based on a graphite epoxy-composite (GECE-Tyr) showing a sensitivity of 294 µA/mM cm2, a detection limit of 0.01 mM for a signal-to-noise ratio of 3 in a concentration range of 0.0–0.15 mM catechol with a response time of 20 s and an RSD of 8% (n = 3). The electrodes were stable for more than 24 h. A 90% increase of the signal indicated that the response is better with the biocomposite based on carbon nanotubes rather than with the graphite.

Graphical abstract: Improvement of the electrochemical detection of catechol by the use of a carbon nanotube based biosensor

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Article information

DOI
https://doi.org/10.1039/B808387H
Article type
Paper
Submitted
19 May 2008
Accepted
22 Aug 2008
First published
18 Oct 2008

Analyst, 2009,134, 60-64

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Improvement of the electrochemical detection of catechol by the use of a carbon nanotube based biosensor

B. Pérez López and A. Merkoçi, Analyst, 2009, 134, 60 DOI: 10.1039/B808387H

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