Mineral substituted hydroxyapatite coatings deposited on nanoporous TiO2 modulate the directional growth and activity of osteoblastic cells

Dharman Govindaraj; Mariappan Rajan; Murugan A. Munusamy; Akon Higuchi

doi:10.1039/C5RA11037H

Mineral substituted hydroxyapatite coatings deposited on nanoporous TiO₂ modulate the directional growth and activity of osteoblastic cells†

Dharman Govindaraj,^a Mariappan Rajan,*^a Murugan A. Munusamy^b and Akon Higuchi^bc

* Corresponding authors

^a Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, India
E-mail: rajanm153@gmail.com
Tel: +91 9488014084

^b Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia

^c Department of Chemical & Materials Engineering National Central University, No. 300 Jung da Rd., Chung-Li, Taoyuan 320, Taiwan, Republic of China

Abstract

The biocompatibility of anodized titanium (TiO₂) was improved by electrophoretically deposited mineral (strontium (Sr), magnesium (Mg) and zinc (Zn)) substituted hydroxyapatite (M-HAP). The M-HAP layer was grown on the anodized Ti surface at different deposition temperatures (room temperature, 60 and 80 °C). The phases and morphologies of the M-HAP layers were influenced by the deposition temperature. The coatings were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Scanning electron microscopy equipped with energy dispersive X-ray analysis (SEM-EDX). Also, the effects of temperature and the mineral substitution of Sr, Mg and Zn for Ca on the physiochemical and biological properties of the M-HAP coatings were evaluated by the mechanical strength, ion dissolution and proliferation, alkaline phosphatase (ALP) activity and osteogenic expression of osteoblast like cells MG66 (HOS). Thus, the M-HAP deposition of TiO₂ will serve as a potential candidate for orthopedic applications.

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Article information

DOI: https://doi.org/10.1039/C5RA11037H
Article type: Paper
Submitted: 10 Jun 2015
Accepted: 02 Jul 2015
First published: 02 Jul 2015

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RSC Adv., 2015,5, 58980-58988

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Mineral substituted hydroxyapatite coatings deposited on nanoporous TiO₂ modulate the directional growth and activity of osteoblastic cells

D. Govindaraj, M. Rajan, M. A. Munusamy and A. Higuchi, RSC Adv., 2015, 5, 58980 DOI: 10.1039/C5RA11037H

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