Ce3/4+ cation-functionalized maghemite nanoparticles towards siRNA-mediated gene silencing

Liron L. Israel; Emmanuel Lellouche; Ron S. Kenett; Omer Green; Shulamit Michaeli; Jean-Paul Lellouche

doi:10.1039/C4TB00634H

Ce^3/4+ cation-functionalized maghemite nanoparticles towards siRNA-mediated gene silencing†

Liron L. Israel,^a Emmanuel Lellouche,^b Ron S. Kenett,^cd Omer Green,^a Shulamit Michaeli*^b and Jean-Paul Lellouche*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry & Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel
E-mail: Jean-Paul.M.Lellouche@biu.ac.il

^b Faculty of Life Sciences & Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel
E-mail: shulamit.michaeli@biu.ac.il

^c KPA Ltd., Raanana 43100, Israel

^d University of Turin, Italy

Abstract

Pre-formed Massart magnetite (Fe₃O₄) nanoparticles (NPs) have been successfully modified by positively charged lanthanide Ce(III/IV) cations/[CeL_n]^3/4+ complexes by using a strong mono-electronic Ceric Ammonium Nitrate oxidant (CAN) as a Ce donor. The doping process is promoted by high-power ultrasonic irradiation. The reaction has been statistically optimized by Design of Experiments (DoE, MINITAB® 16 DoE software) to afford globally optimized magnetically responsive ultra-small 6.61 ± 2.04 nm-sized CAN_DOE–γ-Fe₂O₃ NPs that are highly positively charged (ξ potential: +45.7 mV). This innovative inorganic DoE-optimized NP surface modification by [CeL_n]^3/4+ complexes enables an effective “fully inorganic-type” coordination attachment of a branched poly-cationic 25 kDa b-PEI₂₅ polymer for siRNA loading and gene silencing. This innovative NP platform technology paves an efficient way for the successful development of a wide range of biomedicine and diagnostic-related applications.

Supplementary files

Article information

DOI: https://doi.org/10.1039/C4TB00634H
Article type: Paper
Submitted: 22 Apr 2014
Accepted: 20 Jul 2014
First published: 23 Jul 2014

Download Citation

J. Mater. Chem. B, 2014,2, 6215-6225

Author version available

Download author version (PDF)

Permissions

Request permissions

Ce^3/4+ cation-functionalized maghemite nanoparticles towards siRNA-mediated gene silencing

L. L. Israel, E. Lellouche, R. S. Kenett, O. Green, S. Michaeli and J. Lellouche, J. Mater. Chem. B, 2014, 2, 6215 DOI: 10.1039/C4TB00634H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Journal of Materials Chemistry B