Issue 34, 2013
From the journal:

RSC Advances

Gold–silver nanocomposite-functionalized graphene sensing platform for an electrochemiluminescent immunoassay of a tumor marker†

Yan Zhang,a   Long Li,b   Hongmei Yang,a   Ya-nan Ding,a   Min Su,a   Jiantao Zhu,a   Mei Yan,a   Jinghua Yu*a  and  Xianrang Songc  

* Corresponding authors

a Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China
E-mail: ujn.yujh@gmail.com

b Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, P. R. China

c Cancer Research Center, Shandong Tumor Hospital, Jinan 250117, P. R. China

Abstract

A novel electrochemiluminescence (ECL) immunosensor based on a prepared 3D paper device was designed for the sensitive detection of a tumor marker in human serum by using CdTe quantum dot coated carbon microspheres (QD@CMs) as bionanolabels. To construct such an ECL immunosensor, gold–silver nanocomposite-functionalized graphene was prepared and used as a sensing platform, which increased the surface area to capture a large number of primary antibodies as well as improving the electronic transmission rate. With the employment of the as-prepared QD@CMs as bionanolabels, a good ECL performance (excellent precision and sensitivity) was exhibited through a sandwich-type immunoassay. To examine the signal amplification of the immunoassay, the analytical properties of various immunosensor platforms and labels were compared in detail. As a proof-of-concept, cancer antigen 125 was used as a model analyte and satisfactory results were obtained. Therefore, the proposed method provides a new promising platform for clinical immunoassays of other biomolecules.

Graphical abstract: Gold–silver nanocomposite-functionalized graphene sensing platform for an electrochemiluminescent immunoassay of a tumor marker

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3RA41666F
Article type
Paper
Submitted
07 Apr 2013
Accepted
12 Jun 2013
First published
14 Jun 2013

RSC Adv., 2013,3, 14701-14709

Permissions

Request permissions

Gold–silver nanocomposite-functionalized graphene sensing platform for an electrochemiluminescent immunoassay of a tumor marker

Y. Zhang, L. Li, H. Yang, Y. Ding, M. Su, J. Zhu, M. Yan, J. Yu and X. Song, RSC Adv., 2013, 3, 14701 DOI: 10.1039/C3RA41666F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements