Issue 45, 2012
From the journal:

Journal of Materials Chemistry

Porphyrin functionalized graphenenanosheets-based electrochemical aptasensor for label-free ATP detection

Hongfen Zhang,ab   Yujie Han,a   Yujing Guo*a  and  Chuan Dong*a  

* Corresponding authors

a Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan, China
E-mail: guoyj@sxu.edu.cn, dc@sxu.edu.cn
Fax: +86-351-7011011
Tel: +86-351-7011011

b School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China

Abstract

A facile strategy for the preparation of meso-terakis(4-methoxyl-3-sulfonatophenyl) porphyrin (T(4-Mop)PS4)-graphene hybrid nanosheets (TGHNs) was demonstrated for the first time, which combines the features of both graphene (high conductivity and large specific surface area) and porphyrins (photochemical electron-transfer ability and excellent electrochemical activity). The as-obtained TGHNs were characterized by UV-vis spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, atomic force microscopy, transmission electron microscopy and cyclic voltammetry, which confirmed that the porphyrin had been effectively functionalized on the surface of graphene. Combined with the high affinity and specificity of an aptamer, a simple, rapid, sensitive and label-free electrochemical aptasensor was successfully fabricated for adenosine triphosphate (ATP) detection. The proposed TGHN-based aptasensor achieved the direct electron transfer of porphyrin and showed good affinity and specificity towards ATP, which avoided interference such as the introduction of labeled-substances and the [Fe(CN)6]3−/4− probe. The preferable linear range for ATP was from 2.2 nM to 1.3 μM (R = 0.9982) with a detection limit of 0.7 nM.

Graphical abstract: Porphyrin functionalized graphene nanosheets-based electrochemical aptasensor for label-free ATP detection

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Article information

DOI
https://doi.org/10.1039/C2JM35379B
Article type
Paper
Submitted
10 Aug 2012
Accepted
17 Sep 2012
First published
20 Sep 2012

J. Mater. Chem., 2012,22, 23900-23905

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Porphyrin functionalized graphene nanosheets-based electrochemical aptasensor for label-free ATP detection

H. Zhang, Y. Han, Y. Guo and C. Dong, J. Mater. Chem., 2012, 22, 23900 DOI: 10.1039/C2JM35379B

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