Issue 17, 2010
From the journal:

Soft Matter

Monitoring cell detachment on PEG-based thermoresponsive surfaces using TIRF microscopy

Katja Uhlig,a   Erik Wischerhoff,b   Jean-François Lutz,b   André Laschewsky,c   Magnus S. Jaeger,*a   Andreas Lankenaua  and  Claus Duschla  

* Corresponding authors

a Fraunhofer Institute for Biomedical Engineering (IBMT), Am Muehlenberg 13, Potsdam, Germany
E-mail: magnus.jaeger@ibmt.fraunhofer.de
Fax: +49 3 31 581 87 399
Tel: +49 3 31 581 87 305

b Fraunhofer Institute for Applied Polymer Research (IAP), P.O. Box 60 06 51, Potsdam, Germany

c Applied Polymer Chemistry, University of Potsdam, K.-Liebknecht-Strasse 24-25, Potsdam, Germany

Abstract

Recently, we introduced a thermoresponsive copolymer that consists of oligo(ethylene glycol) methacrylate (OEGMA) and 2-(2-methoxyethoxy)ethyl methacrylate (MEO2MA). The polymer exhibited an LCST at 35 °C in PBS buffer and was anchored onto gold substrates using disulfide polymerisation initiators. It allows the noninvasive detachment of adherent cells from their substrate. As the mechanisms that determine the interaction of cells with such polymers are not well understood, we employed Total Internal Reflection Fluorescence (TIRF) microscopy in order to monitor the detachment process of cells of two different types. We identified contact area and average cell–substrate distance as crucial parameters for the evaluation of the detachment process. The sensitivity of TIRF microscopy allowed us to correlate the specific adhesion pattern of MCF-7 breast cancer cells with the morphology of cell deposits that may serve as fingerprints for a nondestructive characterisation of live cells.

Graphical abstract: Monitoring cell detachment on PEG-based thermoresponsive surfaces using TIRF microscopy

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Article information

DOI
https://doi.org/10.1039/C0SM00010H
Article type
Paper
Submitted
19 Feb 2010
Accepted
10 Jun 2010
First published
02 Jul 2010

Soft Matter, 2010,6, 4262-4267

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Monitoring cell detachment on PEG-based thermoresponsive surfaces using TIRF microscopy

K. Uhlig, E. Wischerhoff, J. Lutz, A. Laschewsky, M. S. Jaeger, A. Lankenau and C. Duschl, Soft Matter, 2010, 6, 4262 DOI: 10.1039/C0SM00010H

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