Issue 11, 2010
From the journal:

Analytical Methods

Gold nanoparticles/l-cysteine/graphene composite based immobilization strategy for an electrochemical immunosensor

Guangfeng Wang,ab   Hao Huang,ab   Ge Zhang,ab   Xiaojun Zhang,ab   Bin Fangab  and  Lun Wang*ab  

* Corresponding authors

a College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Key Laboratory of Chem-Biosensing, Anhui Normal UniVersity, Wuhu, P R China
E-mail: wangyusandra@yahoo.com.cn
Fax: +86-553-3869303
Tel: +86-553-3869303

b Anhui Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, Hefei University of Technology, Hefei, P R China

Abstract

A novel immobilization strategy combining gold nanoparticles (GNP) with graphene (Gr) via crosslinker L-cysteine (L-cys) for the enhancement of a HRP-labelled sandwiched electrochemical immunosensor was developed. In the fabrication process of the immobilization platform, graphene was first modified onto glassy carbon electrode (GCE), and then L-cys was electrodeposited onto the substrate of Gr for the following GNP assembling. The characteristics of the platform surface were studied by scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) spectrometry, cyclic voltammetry (CV) and electrochemical alternating current impedance spectroscopy (EIS). From the SEM, interestingly we found the substrate of Gr induced the regular growth of L-cys, which may further improve the stable conjugation of GNP, which may result in more loading amount of the biomolecules. The special GNP/L-cys/Gr composite film constructed an effective antibody immobilization matrix and made the immunosensor hold high sensitivity, good stability and bioactivity. Under the optimized experimental conditions, a wide linear range of human IgG (HIgG) concentration from 0.2 to 320 ng mL−1 and a detection limit of 70 ng mL−1 were obtained. Accurate detection of HIgG in human serum samples was satisfyingly demonstrated by comparison to the standard ELISA assays.

Graphical abstract: Gold nanoparticles/l-cysteine/graphene composite based immobilization strategy for an electrochemical immunosensor

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Article information

DOI
https://doi.org/10.1039/C0AY00389A
Article type
Paper
Submitted
20 Jun 2010
Accepted
15 Aug 2010
First published
24 Sep 2010

Anal. Methods, 2010,2, 1692-1697

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Gold nanoparticles/L-cysteine/graphene composite based immobilization strategy for an electrochemical immunosensor

G. Wang, H. Huang, G. Zhang, X. Zhang, B. Fang and L. Wang, Anal. Methods, 2010, 2, 1692 DOI: 10.1039/C0AY00389A

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