Issue 8, 2010
From the journal:

Journal of Materials Chemistry

Fluorescent nanoparticles for ratiometric pH-monitoring in the neutral range†

Anja Schulz,ab   Jana Wotschadlo,c   Thomas Heinzec  and  Gerhard J. Mohr*ad  

* Corresponding authors

a Institute of Physical Chemistry, Friedrich-Schiller-Universität Jena, Lessingstraße 10, Jena, Germany
E-mail: gerhard.mohr@uni-jena.de
Fax: +49 3641 948302
Tel: +49 3641 948368

b Optical Sensors Laboratory, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland
E-mail: anja.schulz@dcu.ie

c Center of Excellence for Polysaccharide Research, Friedrich-Schiller-Universität Jena, Humboldtstraße 10, Jena, Germany
E-mail: thomas.heinze@uni-jena.de, jana.wotschadlo@uni-jena.de

d Fraunhofer Institute for Reliability and Microintegration (IZM), Department of Polytronic Systems, Workgroup Sensor Materials, Josef-Engert-Straße 9, Regensburg, Germany

Abstract

This article describes the synthesis and characterisation of poly(acrylamide) nanoparticles (NPs) with a covalently immobilised pH-sensitive fluorophore. With a pKa of 6.7, good photostability, emission in the visible spectral range and large Stokes' shift the naphthalimide-based pH-indicator is well suited for bio-analytical applications. Additionally, the nanosensors contain a pH-insensitive reference fluorophore that allows the ratiometric monitoring of pH e.g. inside living cells. In order to enhance the cellular uptake and endosomal escape of the beads, novel core–shell nanoparticles bearing a positive ζ-potential are synthesised and evaluated.

Graphical abstract: Fluorescent nanoparticles for ratiometric pH-monitoring in the neutral range

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Article information

DOI
https://doi.org/10.1039/B918427A
Article type
Paper
Submitted
04 Sep 2009
Accepted
27 Nov 2009
First published
05 Jan 2010

J. Mater. Chem., 2010,20, 1475-1482

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Fluorescent nanoparticles for ratiometric pH-monitoring in the neutral range

A. Schulz, J. Wotschadlo, T. Heinze and G. J. Mohr, J. Mater. Chem., 2010, 20, 1475 DOI: 10.1039/B918427A

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