Issue 5, 2005
From the journal:

Dalton Transactions

Detection of secondary structures in 17-mer Ru(ii)-labeled single-stranded oligonucleotides from luminescence lifetime studies†

D. García-Fresnadillo,a   O. Lentzen,b   I. Ortmans,b   E. Defrancqc  and  A. Kirsch-De Mesmaeker*b  

* Corresponding authors

a Department of Organic Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, Avenida Complutense s/n, E-28040 Madrid, Spain
E-mail: dgfresna@quim.ucm.es
Fax: +34 91394 4103
Tel: +34 91394 4220

b Université Libre de Bruxelles, Organic Chemistry and Photochemistry, CP 160/08, 50 Avenue F. D. Roosevelt, B-1050 Brussels, Belgium
E-mail: akirsch@ulb.ac.be
Fax: +32 2 650 30 18
Tel: +32 2 650 30 17

c LEDSS, UMR CNRS 5616, ICMG FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France
E-mail: Eric.Defrancq@ujf-grenoble.fr
Fax: +33(0)4 76 51 49 46
Tel: +33(0)4 76 51 44 33

Abstract

The emission properties of a non intercalating complex, [Ru(TAP)2(dip)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene; dip = 4,7-diphenyl-1,10-phenanthroline), tethered to 17-mer single-stranded oligodeoxyribonucleotides (ODNs) either in the middle or at the 5′-end of the sequence, are determined. The results highlight the fact that the luminescence of this metallic compound is sufficiently sensitive to its microenvironment to probe self-structuration of these short single-stranded ODNs. It is shown that the weighted averaged emission lifetimes (τM) along with the quenching rate constants of luminescence by oxygen reflect particularly well different structures adopted by the different ODNs sequences. The determination of these parameters thus offers an elegant way to examine possible structurations of synthetic single-stranded ODNs that play important roles in biological applications.

Graphical abstract: Detection of secondary structures in 17-mer Ru(ii)-labeled single-stranded oligonucleotides from luminescence lifetime studies

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Article information

DOI
https://doi.org/10.1039/B415898A
Article type
Paper
Submitted
14 Oct 2004
Accepted
13 Jan 2005
First published
01 Feb 2005

Dalton Trans., 2005, 852-856

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Detection of secondary structures in 17-mer Ru(II)-labeled single-stranded oligonucleotides from luminescence lifetime studies

D. García-Fresnadillo, O. Lentzen, I. Ortmans, E. Defrancq and A. Kirsch-De Mesmaeker, Dalton Trans., 2005, 852 DOI: 10.1039/B415898A

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