Issue 5, 2004
From the journal:

Analyst

Matrix effects in immunobiosensor determination of clenbuterol in urine and serum

M. Annette Johansson*ab  and  Karl-Erik Hellenäsa  

* Corresponding authors

a National Food Administration, PO Box 622, 751 26 Uppsala, Sweden
E-mail: ajoh@slv.se

b Swedish University of Agricultural Sciences, Department of Food Science, PO Box 7051, 750 07 Uppsala, Sweden

Abstract

The construction of immunochemical inhibition assays for β-agonist and hormone residues have previously been described. In the present work the β-agonist assay was further optimised for application to biological samples, using urine as the main model matrix. Matrix interferences with the antigen–antibody interaction and non-specific binding (NSB) of matrix components to the sensor surface were systematically studied. A full factorial design experiment was employed for evaluating the effects of assay buffer composition. In addition, the influence of antibody concentration and sample dilution on the matrix background was investigated. NSB from urine was highly affected by buffer pH and salt concentration, while buffer composition had little effect on matrix interferences with the antigen–antibody interaction. Ultra-filtration efficiently prevented NSB from urine and serum samples. Increased antibody dilution reduced the matrix background while sample dilution had an opposite effect.

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Article information

DOI
https://doi.org/10.1039/B316723B
Article type
Paper
Submitted
23 Dec 2003
Accepted
18 Mar 2004
First published
01 Apr 2004

Analyst, 2004,129, 438-442

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Matrix effects in immunobiosensor determination of clenbuterol in urine and serum

M. A. Johansson and K. Hellenäs, Analyst, 2004, 129, 438 DOI: 10.1039/B316723B

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