Issue 10, 2001
From the journal:

Analyst

Amperometric tyrosinase biosensor based on a sol–gel-derived titanium oxide–copolymer composite matrix for detection of phenolic compounds

Xu Chen,a   Guangjin Chenga  and  Shaojun Dong*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
E-mail: dongsj@ns.ciac.jl.cn;
Fax: +86 431 5689711

Abstract

A new type of tyrosinase biosensor was developed for the detection of phenolic compounds, based on the immobilization of tyrosinase in a sol–gel-derived composite matrix that is composed of titanium oxide sol and a grafting copolymer of poly(vinyl alcohol) with 4-vinylpyridine. Tyrosinase entrapped in the composite matrix can retain its activity to a large extent owing to the good biocompatibility of the matrix. The parameters of the fabrication process and the variables of the experimental conditions for the enzyme electrode were optimized. The resulting sensor exhibited a fast response (20 s), high sensitivity (145.5 μA mmol−1 l) and good storage stability. A detection limit of 0.5 μM catechol was obtained at a signal-to-noise ratio of 3.

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B103787K
Article type
Paper
Submitted
26 Apr 2001
Accepted
13 Jul 2001
First published
05 Sep 2001

Analyst, 2001,126, 1728-1732

Permissions

Request permissions

Amperometric tyrosinase biosensor based on a sol–gel-derived titanium oxide–copolymer composite matrix for detection of phenolic compounds

X. Chen, G. Cheng and S. Dong, Analyst, 2001, 126, 1728 DOI: 10.1039/B103787K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements