Abstract
In common with certain other lymphoid neoplasms, cells of the human lymphocytic leukemia lines 1873 and 1929 are asparagine (ASN) auxotrophs. Asparagine synthetase (ASY), which is a housekeeping gene, is repressed and the promoting region of the gene is highly methylated. We now demonstrate in these cells multiple levels in control of the expression of this gene, in a system of cocultivation with macrophages and other cell types. In this system, mediated by cell-to-cell contact, ASY becomes expressed by the leukemic cells and they become prototrophic. Demethylation of ASY occurs; it follows expression and is permanent over multiple cell generations, but the cells return to auxotrophy with rapid repression of ASY on removal from cell contact. With ASY expression, the associated histone H3 at lysine position 9 (H3K9) becomes acetylated and H3K4, methylated. In contrast to other systems, H3K9 methylation does not characterize the repressed state. The changes leading from repression to induction of ASY and demethylation parallel the physiological changes specific to functional maturation of normal lymphoid precursors. The lability of expression of ASY has potential significance in determining the sensitivity of leukemic cells to L-asparaginase.
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References
Broome JD . L-asparaginase: discovery and development as a tumor-inhibitory agent. Cancer Treat Rep 1981; 65 (Suppl 4): 111–114.
Pinheiro JP, Boos J . The best way to use asparaginase in childhood acute lymphatic leukaemia-still to be defined? Br J Haematol 2004; 125: 117–127.
Patterson MK, Orr GR . L-asparagine biosynthesis by nutritional variants of the Jensen sarcoma. Biochem Biophys Res Comm 1967; 26: 228–233.
Broome JD . Studies on the mechanism of tumor inhibition by L-asparaginase. J Exp Med 1968; 127: 1055–1072.
Peng H, Shen N, Qian L, Sun XL, Goodwin LO, Issa JP et al. Hypermethylation of the CpG islands in the mouse asparagine synthetase gene: relarionship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells. Br J Cancer 2001; 85: 930–935.
Ray PN, Siminovitch L, Andrulis IL . Molecular cloning of a cDNA for Chinese hamster ovary asparagine synthetase. Gene 1984; 30: 1–9.
Andrulis IL, Chen J, Ray PN . human cDNA for asparagine synthetase and expression in Jensen sarcoma cells. Mol Cell Biol 1987; 7: 2435–2443.
Greco A, Gong SS, Ittman M, Basilico C . Organization and expression of the cell cycle gene, ts11, that encodes asparagine synthetase. MolCell Biol 1989; 9: 2350–2359.
Zhong C, Chen C, Kilberg MS . Characterization of the nutrient-sensing response unit in the human asparagine synthetase promoter. Biochem J 2003; 372: 603–609.
Bird A . DNA methylation patterns and epigenetic memory. Genes Dev 2002; 16: 6–21.
Li E . Chromatin modification and epigenetic reprogrammong in mammalian development. Nat Rev Genet 2002; 3: 662–673.
Ren Y, Roy S, Ding Y, Iqbal J, Broome JD . Methylation of the asparagine synthetase promoter in human leukemic cell lines is associated with a specific methyl binding protein. Oncogene 2004; 23: 3953–3961.
Narlikar GJ, Fan HY, Kingston RE . Cooperation between complexes that regulate chromatin structure and transcription. Cell 2002; 108: 475–487.
Strahl BD, Allis CD . The language of covalent histone modifications. Nature 2000; 403: 41–45.
Lachner M, O'Sullivan RJ, Jenuwein T . An epigenetic road map for histone lysine methylation. J Cell Sci 2003; 116: 2117–2124.
Drexler HG . Leukemic-Lymphoma Cell Line Facts Book. San Diego: Academic Press, 2001, pp 33–45.
Coombes MM, Briggs KL, Bone JR, Clayman GL, El-Naggae AK, Dent SY . Resetting the histone code at CDKN3A in HNSCC by inhibition of DNA methylation. Oncogene 2003; 22: 8902–8911.
Chim CS, Wong AS, Kwong YL . Epigenetic inactivation of INK4/CDK/RB cell cycle pathway in acute leukemias. Ann Hematol 2003; 82: 738–742.
Hall BM, Gibson LF . Regulation of lymphoid and myeloid leukemic cell survival: role of stromal cell adhesion molecules. Leuk Lymphoma 2004; 45: 35–48.
Murti KG, Brown PS, Kumagai M, Campana D . Molecular interactions between human B-cell progenitors and the bone marrow microenvironment. Exp Cell Res 1996; 226: 47–58.
Avner P, Heard E . X-chromosome inactivation: counting, choice and initiation. Nat Rev Genet 2001; 2: 59–67.
Mutskov V, Felsenfeld G . Silencing of transgene transcription precedes methylation of promoter DNA and histone H4 lysine 9. EMBO J 2004; 23: 138–149.
Turker MS . Gene silencing in mammalian cells and the spread of methylation. Oncogene 2002; 21: 5388–5393.
Herman JG, Baylin SB . Gene silencing in cancer in association with promoter hypermethylation. N Engl J Med 2003; 349: 2042–2054.
Lee DU, Agarwal S, Rao A . Th2 lineage commitment and efficient IL-4 production involves extended demethylation of the IL-4 gene. Immunity 2002; 16: 649–660.
Saccani S, Natoli G . Dynamic changes in histone H3 Lys 9 methylation occurring at tightly regulated inducible inflammatory genes. Genes Dev 2002; 16: 2219–2224.
Richards EJ, Elgin DC . Epigenetic codes for heterochromatin formation and silencing: rounding up the usual suspects. Cell 2002; 108: 498–500.
Mullen AC, Hutchins AS, High FA, Lee HW, Sykes KJ, Chodosh LA et al. Hlx is induced by and genetically interacts with T-bet to promote heritable T(H)1 gene induction. Nat Immunol 2003; 3: 652–658.
Bachman KE, Park BH, Rhee I, Rajagopalan H, Herman JG, Baylin SB et al. Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene. Cancer Cell 2003; 3: 89–95.
Pui CH, Relling MV, Downing JR . Acute lymphoblastic leukemia. N Engl J Med 2004; 350: 1535–1548.
Grundman E, Oettgen HF . Experimental and clinical effects of L-asparaginase. Rec Results Cancer Res 1970; 33: 219–235.
Krejci O, Starkova J, Otova B, Madzo J, Kalinova M, Hrusak O et al. Upregulation of asparagine synthetase fails to avert cell cycle arrest induced by L-asparaginase in TEL/AML1-positive leukemic cells. Leukemia 2004; 18: 434–441.
Stams WA, der Boer ML, Beverloo HB, Meijerink JP, Stigter RL, van Wering ER et al. Sensitivity to L-asparaginase is not associated with expression levels of asparagine synthetase in t(12;21)+ pediatric ALL. Blood 2003; 101: 2743–2747.
Hope KJ, Jin L, Dick JE . Acute myeloid leukemia originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity. Nat Immunol 2004; 5: 738–743.
Sell S . Stem cell origin of cancer and differentiation therapy. Crit Rev Oncol Hematol 2004; 51: 1–28.
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This paper is written in remembrance of Dr John G Kidd (1908–1991).
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Ding, Y., Li, Z. & Broome, J. Epigenetic changes in the repression and induction of asparagine synthetase in human leukemic cell lines. Leukemia 19, 420–426 (2005). https://doi.org/10.1038/sj.leu.2403639
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DOI: https://doi.org/10.1038/sj.leu.2403639
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