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Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression

Nature Genetics volume 2, pages 173–179 (1992)Cite this article

Abstract

Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large–scale sequencing of a 3′–directed cDNA library from the human liver cell line HepG2, that is a non–biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for protein–synthesis. The abundance of cDNA species varied from 2.2% to less than 0.004%. Fifty two percent of the mRNA were abundant species consisting of 173 genes and the rest were non–abundant, consisting of about 6,600 genes.

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Authors and Affiliations

  1. Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamada-oka, Suita, Osaka, 565, Japan

    Kousaku Okubo, Naohiro Hori, Ryo Matoba, Toshiyuki Niiyama, Atsushi Fukushima, Yuko Kojima & Kenichi Matsubara

Authors
  1. Kousaku Okubo
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  2. Naohiro Hori
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  3. Ryo Matoba
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  4. Toshiyuki Niiyama
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  5. Atsushi Fukushima
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  6. Yuko Kojima
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  7. Kenichi Matsubara
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Okubo, K., Hori, N., Matoba, R. et al. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nat Genet 2, 173–179 (1992). https://doi.org/10.1038/ng1192-173

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