Abstract
Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double–strand breaks of DNA. We have cloned genes from human, mouse and fission yeast that are homologous to rad51. The 339 amino acid proteins predicted for the two mammalian genes are almost identical and are highly homologous (83%) with the yeast proteins. The mouse gene is transcribed at a high level in thymus, spleen, testis andpvary and at a lower level in brain and other tissues. The rad51 homologues fail to complement the DNA repair defect of rad51 mutants of S. cerevisiae. The mouse gene is located in the F1 region of chromosome 2 and the human gene maps to chromosome 15.
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References
Horii, T., Ogawa, T. & Ogawa, H. Organization of the recA gene of Escherichia coli. Proc. natn. Acad. Sci. U.S.A. 77, 313–317 (1980).
Masukata, H., Fujii, T., Ogawa, T. & Ogawa, H. Biologically active recombinant formed through DNA pairing by purified recA protein in vitro. Molec. gen. Genet. 189, 226–234 (1983).
West, S. Enzymes and molecular mechanisms of genetic recombination. Ann. Rev. Biochem. 61, 603–640 (1992).
Tomizawa, J. & Ogawa, H. Breakage of DNA in Rec+ and Rec− bacteria by disintegration of radiophosphorous atoms in DNA and possible cause of pleiotropic effects of RecA mutation. Cold Spring Harb. Symp. quant Biol. 33, 243–251 (1968).
Jachymczyk, W.J., Von Borstel, R.C., Mowat, M.R.A. & Hastings, P.J. Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: The RAD3 system and the RAD51 system. Molec. gen. Genet. 182, 196–205 (1981).
Shinohara, A., Ogawa, H. & Ogawa, T. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell 69, 457–470 (1992).
Bishop, D.K., Park, D., Xu, L. & Kleckner, N. DMC1: A meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell 69, 439–456 (1992).
Ogawa, T., Yu, X., Shinohara, A. & Egelman, E.H. Similarity of the Yeast Rad51 Filament to the Bacterial RecA Filament. Science 259, 1896–1899 (1993).
Kozak, M. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucl. Acids Res. 16, 8126–8148 (1987).
Bezzubova, O., Shinohara, A., Mueller, R.G., Ogawa, H. & Buerstedde, J.-M. A chicken RAD51 homologue is expressed at high levels in lymphoid and reproductive organs. Nucl. Acids Res. 21, 1577–1580 (1993).
Kobayashi, T., Hotta, Y. & Tabata, S. Isolation and characterization of a yeast gene which is homologous with a meiosis specific cDNA from a plant. Molec. gen. Genet. 237, 225–232 (1993).
Matsuda, Y. et al. Location of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding. Cytogenet. cell Genet. 61, 282–285 (1992).
Siracusa, L.D. & Abbott, C.M. Chromosome 2. Mamm. Genome 3, S20–S43 (1992).
Story, R., Weber, I.T. & Steitz, T.A. The structure of the E. coli recA protein monomer and polymer. Nature 355, 318–325 (1992).
Benedict, R.C. & Kowalczykowski, S.C. Increase of the DNA strand assimilation activity of recA protein by removal of the C-terminus and structure-function studies of the resulting protein fragment. J. biol. Chem. 263, 15513–15520 (1988).
Yu, X. & Egelman, E.H. Removal of the RecA C-terminus results in a conformational change in the RecA-DNA filament. J. struct. Biol. 106, 243–254 (1991).
Tateishi, S., Horii, T., Ogawa, T. & Ogawa, H. C-terminal truncated Escherichia coli RecA protein RecA5327 has enhanced binding affinities to single- and double-stranded DNAs. J. molec. Biol. 223, 115–129 (1992).
Tonegawa, S. Somatic generation of antibody diversity. Nature 302, 575–581 (1983).
Schatz, D.G., Oettinger, M.A. & Schissel, M.S. V(D)J recombination: Molecular biology and regulation. Ann. Rev. Immunol. 10, 359–383 (1992).
Lutzker, S.G. & Alt, F.W. Immunoglobulin heavy-chain class switching. In The Mobile DNA (eds Berg, D.E. & Howe, M.M.) 693–714 (American Society of Microbiology, Washington, 1989).
Basile, G., Aker, M. & Mortimer, R.T. Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51. Molec. cell. Biol. 12, 3235–3246 (1992).
Resnick, M.A. Investigating the genetic control of biochemical events in meiotic recombination. In The Meiosis (ed. Moens, P.B.) 157–210 (Academic Press, New York, 1987).
Lewin, B. Commitment and activation at Pol II promoters: A tail of protein-protein interactions. Cell 61, 1161–1164 (1990).
Gill, G. & Tjian, R. A highly conserved domain of TFIID displays species specificity in vivo. Cell 65, 333–340 (1991).
Cormack, B.P., Strubin, M., Ponticelli, A.S. & Struhl, K. Functional differences between yeast and human TFIID are localized to highly conserved region. Cell 65, 341–348 (1991).
Sanger, F., Nicklen, S. & Coulson, A.R. DNA sequencing with chain-terminating inhibitors. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Feinberg, A.P. & Vogelstein, B. A technique for radiolabelling DNA restriction endonuclease fragment to high specific activity. Anal. Biochem. 132, 6–13 (1983).
Kunkel, T.A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. natn. Acad. Sci. U.S.A. 82, 488–492 (1985).
Nei, M. Molecular Population Genetics and Evolution (North-Holland/American. Elsevier, Amsterdam, 1975).
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Shinohara, A., Ogawa, H., Matsuda, Y. et al. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet 4, 239–243 (1993). https://doi.org/10.1038/ng0793-239
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DOI: https://doi.org/10.1038/ng0793-239
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