Abstract
IN recent years, many episomes other than the F factor of E. coli K12 have been discovered, such as the colicinogenic factors and the drug resistance transfer factors. Some of these seem to be related to F since they determine a pilus similar to the F pilus in serological properties and male specific phage sensitivity1. These F-like plasmids frequently inhibit their own transfer and of F when present in the same cell, but mutants have been obtained which do not produce the inhibiting substance and which allow transfer at a high rate1,2. One such mutant, R100-1, was used in the work to be described here.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Meynell, E., Meynell, G. G., and Datta, N., Bact. Rev., 32, 55 (1968).
Egawa, R., and Hirota, Y., Japan. J. Genet., 37, 66 (1962).
Hirota, Y., Fujii, T., and Nishimura, Y., J. Bact., 91, 1298 (1966).
Ohtsubo, E., Nishimura, Y., and Hirota, Y., Genetics, 64, 173 (1970).
Willetts, N. S., and Broda, P. M. A., Bacterial Episomes and Plasmids, Ciba Foundation Symp., 32 (1969).
Achtman, M., Willetts, N. S., and Clark, A. J., Genetics, 60, 157 (1968).
Brinton, C. C., Trans. NY Acad. Sci., 27, 1003 (1965).
Caro, L. G., and Schnös, M., Proc. US Nat. Acad. Sci., 56, 126 (1966).
Willetts, N. S., and Finnegan, D. J., Genet. Res. (in the press)
Romero, E., and Meynell, E., J. Bact., 97, 780 (1969).
Nishimura, Y., Ishibashi, M., Meynell, E., and Hirota, Y., J. Gen. Microbiol., 49, 89 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WILLETTS, N. Plasmid Specificity of Two Proteins required for Conjugation in E. coli K12. Nature New Biology 230, 183–185 (1971). https://doi.org/10.1038/newbio230183a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/newbio230183a0
This article is cited by
-
Transfer-deficient cointegrates of Flac and lambda prophage
Molecular and General Genetics MGG (1980)
-
Transposon 10 promoted deletions and inversions in the transfer genes of R100-1
Molecular and General Genetics MGG (1979)
-
Investigations of the F conjugation gene traI:traI mutants and λtraI transducing phages
Molecular and General Genetics MGG (1979)
-
tra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5
Molecular and General Genetics MGG (1978)
-
Characterisation of transfer-deficient mutants of the R100-1TcS plasmid pDU202, caused by insertion of Tn10
Molecular and General Genetics MGG (1977)