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A fiber-optic DNA biosensor microarray for the analysis of gene expression

Nature Biotechnology volume 14, pages 1681–1684 (1996)Cite this article

Abstract

A fiber-optic biosensor array is described for the simultaneous analysis of multiple DNA sequences. A bundle of optical fibers was assembled with each fiber carrying a different oligonucleotide probe immobilized on its distal end. Hybridization of fluorescently labeled complementary oligonucleotides to the array was monitored by observing the increase in fluorescence that accompanied binding. The approach enables fast (<10 min) and sensitive (10 nM) detection of multiple DNA sequences simultaneously, with the potential for quantitative hybridization analysis.

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Author information

Author notes

  1. David R. Walt: dwalt@pearl. tufts.edu

Authors and Affiliations

  1. The Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, MA, 02155

    Jane A. Ferguson

  2. Mosaic Technologies, 800 Huntingdon Ave., Boston, MA, 02115

    T. Christian Boles & Christopher P. Adams

Authors
  1. Jane A. Ferguson
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  2. T. Christian Boles
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  3. Christopher P. Adams
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  4. David R. Walt
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Ferguson, J., Boles, T., Adams, C. et al. A fiber-optic DNA biosensor microarray for the analysis of gene expression. Nat Biotechnol 14, 1681–1684 (1996). https://doi.org/10.1038/nbt1296-1681

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  • DOI: https://doi.org/10.1038/nbt1296-1681

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