Abstract
Grapes, the world's most widely-grown fruit crop, are difficult to breed by conventional methods. Here we report a first step in the application of biotechnology to the genetic improvement of this crop, the development of methods for genetic transformation. Co-cultivation of hypocotyl ex-plants from somatic embryos of the root-stock, Rupestris St. George (Vitis rupestris), with a disarmed Agrobacterium tumefaciens strain encoding β-glucuronidase (GUS) and kanamycin resistance, followed by culture on a regeneration medium containing kanamycin, led to formation of transgenic buds and transgenic plants. Petiole explants of the Vitis vinifera cultivars, Cabernet Sauvignon and Ghardonnay, treated similarly gave only transgenic buds.
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References
Mullins, M.G. 1987. Propagation and genetic improvement of temperate fruits: The role of tissue culture, p. 395–405. In: Plant Tissue and Cell Culture, C.E. Green, D.A. Somers, W.P. Hachet and D.D. Biesboer (Eds.), Alan R. Liss, Inc., New York.
Levadoux, L. 1953. De l'origine de la vigne dans les Gaules. Progr. Agric. Vitic. 70: 118–122.
Bouquet, A. 1982. Origine et evolution de l'encépagement français a travers les siècles. Progr. Agric. Vitic. 99: 110–120.
Pongracz, D.P. 1983. Rootstocks for grapevines, p. 150 David Philip Publisher, Cape Town, Johannesburg, London.
Dandekar, A.M., Gupta, P.K., Durzan, D.J. and Knauf, V. 1987. Transformation and foreign gene expression in micropropagated Douglas fir (Pseudotsuga menziesii). Bio/Technology 5: 587–590.
Karnosky, D.F., Diner, A.M. and Barnes, W.M. 1988. A model system for gene transfer in conifers: European larch and Agrobacterium, p. 55–63. In: Somatic Cell Genetics of Woody Plants. M. R. Ahuja (Ed.). Kluwer Academic Publishers, Dordrecht/Boston/London.
Mackay, J., Seguin, A. and Lalonde, M. 1988. Genetic transformation of nine in vitro clones of Alnus and Betula by Agrobacterium tumefaciens. Plant Cell Reports 7: 229–232.
Vahala, T., Stabel, T. and Eriksson, T. 1989. Genetic transformation of willows (Salix spp.) by Agrobacterium tumefaciens. Plant Cell Reports 8: 55–58.
Kobayashi, S. and Uchimiya, H. 1989. Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer. Japan J. Genet. 64: 91–99.
Baribault, T.J., Skene, K.G.M. and Scott, N.S. 1989. Genetic transformation of grapevine cells. Plant Cell Reports 8: 137–140.
Guellec, V., David, C., Branchard, M. and Tempé, J. 1990. Agrobacterium rhizogenes mediated transformation of grapevine (Vitis vinifera L.). Plant Cell, Tissue and Organ Culture 20: 211–215.
Fillatti, J.J., Selmer, J., McCown, B., Haissig, B. and Comai, L. 1987. Agrobacterium-mediated transformation and regeneration of Populus. Mol. Gen. Genet. 206: 192–199.
McGranahan, G.H., Leslie, C.A., Uratsu, S.L., Martin, L.A. and Dandekar, A.M. 1988. Agrobacterium-mediated transformation of walnut somatic embryos and regeneration of transgenic plants. Bio/Technology 6: 800–804.
James, D.J., Passey, A.J., Barbara, D.J. and Bevan, M. 1989. Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector. Plant Cell Reports 7: 658–661.
Bennett, R.K. 1988. Studies on the development of systems for the genetic transformation of crop plants, p. 156. Msc. Agr. Thesis, University of Sydney, Australia.
Barlass, M. and Skene, K.G.M. 1978. In vitro propagation of grapevine (Vitis vinifera) from fragmented shoot apices. Vitis 17: 335–340.
Barlass, M. and Skene, K.G.M. 1980. Studies on the fragmented shoot apex of grapevine. I. The regenerative capacity of leaf primordial fragments in vitro. J. Exp. Bot. 31: 483–488.
Vilaplana, M. and Mullins, M.G. 1989. Regeneration of grapevines (Vitis spp.) in vitro: Formation of adventitious buds on hypocotyls and cotyledons of somatic embryos. J. Plant Physiology 134: 413–419.
Chilton, M.-D. 1988. Plant genetic engineering: progress and promise. J. Agric. Food Chem. 36: 3–5.
Gasser, C.S. and Fraley, R.T. 1989. Genetically engineering crop plants for crop improvement. Science 244: 1293–1299.
Flavell, R.B. 1989. Plant biotechnology and its application to agriculture. Phil. Trans. Roy. Soc. Lond. B. 324: 525–535.
Klee, H.J., Horsch, R.B. and Rogers, S.G. 1987. Agrobacterium-mediated plant transformation and its further applications to plant biology. Annu. Rev. Plant Physiol. 38: 467–486.
Medford, J. and Klee, H. 1989. Manipulation of endogenous auxin and cytokinin levels in transgenic plants, p. 274–XXX In: The Molecular Basis of Plant Development, R. Goldberg (Ed.). Alan R. Liss, Inc., New York.
Mullins, M.G. 1966. Test-plants for investigations of physiology of fruiting in Vitis vinifera L. Nature 209: 419–420.
Mullins, M.G. and Rajasekaran, K. 1981. Fruiting cuttings: revised method of producing test-plants of grapevine cultivars. Amer. J. Enol. Vitic. 32: 35–40.
Rajasekaran, K. and Mullins, M.G. 1979. Embryos and plantlets from cultured anthers of hybrid grapevines. J. Exp. Bot. 30: 399–407.
Nitsch, J.P. and Nitsch, C. 1969. Haploid plants from pollen grains. Science 163: 85–87.
Jouanin, L., Vilaine, F., d'Enfert, C., Casse-Delbart, F. 1985. Localization and restriction maps of the replication origin regions of the plasmids for Agrobacterium rhizogenes strain A4. Mol. Gen. Genet. 201: 370–374.
McBride, K.E. and Summerfelt, K.R. 1990. Improved binary vectors for Agrobacterium-mediated plant transformation. Plant. Mol. Biol. 14: 269–276.
Parsons, T.J., Sinkar, V.P., Stettler, R.F., Nester, E.W. and Gordon, M.P. 1986. Transformation of poplar by Agrobacterium tumefaciens. Bio/Technology 4: 533–536.
Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biol. Reporter 5: 387–405.
Reiss, B., Sprengel, R., Will, H. and Schaller H. 1984. A new sensitive method for qualitative and quantitative assay of neomycin phospho-transferase in crude cell extracts. Gene 30: 211–218.
Irvine, J.M., Oakes, J.V., Shewmaker, C.K. and Crossway, A. 1990. A rapid screen for the detection of specific DNA sequences in plants. Gene Anal. Techn. Appl. 7: 25–31.
Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–507.
Galau, G.A., Bass, H.W. and Hughes, D.W. 1988. Restriction fragment length polymorphisms in diploid and allotetraploid Gossypium: Assigning the late embryogenesis-abundant (Lea) alloalleles in G. hirsutum. Mol. Gen. Genet. 211: 305–314.
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Mullins, M., Tang, F. & Facciotti, D. Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.. Nat Biotechnol 8, 1041–1045 (1990). https://doi.org/10.1038/nbt1190-1041
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DOI: https://doi.org/10.1038/nbt1190-1041
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