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An enzyme-linked oligonucleotide assay

Nature Biotechnology volume 14, pages 1021–1025 (1996)Cite this article

Abstract

The recent development of in vitro methods to select high-affinity ligands by combinatorial chemistry methodologies promises unique and theoretically unlimited supplies of novel therapeutic and diagnostic reagents. One such combinatorial chemistry process, systematic evolution of ligands by exponential enrichment (SELEX), allows rapid identification, from large random sequence pools, of the few oligonucleotide sequences that bind to a desired target molecule with high affinity and specificity. We describe an enzyme-linked sandwich assay that uses a SELEX-derived oligonucleotide. This assay demonstrates that these oligonucleotides can be effective and useful analytical reagents.

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Author notes

  1. Daniel W. Drolet: e-mail: ddrolet@nexstar.com

Authors and Affiliations

  1. NeXstar Pharmaceuticals, 2860 Wilderness Place, Boulder, CO, 80301, USA

    Daniel W. Drolet, Lotus Moon-McDermott & Timothy S. Romig

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Drolet, D., Moon-McDermott, L. & Romig, T. An enzyme-linked oligonucleotide assay. Nat Biotechnol 14, 1021–1025 (1996). https://doi.org/10.1038/nbt0896-1021

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