Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • In the Laboratory
  • Published:

Methods for extracting and amplifying genomic DNA isolated from frozen serum

Nature Biotechnology volume 16, pages 91–94 (1998)Cite this article

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Passos-Bueno, M.R. et al. 1993 Genetic heterogeneity for Duchenne-like muscular dystrophy (DLMD) based on linkage and 50 DAG analysis. Hum. Mol. Genet. 2: 1945–1947.

    Article  CAS  Google Scholar 

  2. Ziv, Y. et al. 1992 Ataxia telangiectasia: kinkage analysis in highly inbred Arab and Druze families and differentiation from an ataxia-microcephaly-cataract syndrome. Hum. Genet. 88: 619–626.

    Article  CAS  Google Scholar 

  3. Hanaoka, Y. and Minaguchi, K. 1996 Sex determination from blood and teeth by PCR amplification of the alphoid satellite family. J. Forensic Sci. 41: 855–858.

    Article  CAS  Google Scholar 

  4. Norby, S. 1993 Screening for the two most frequent mutations in Leber's hereditary optic neuropathy by duplex PCR based on allele-specific amplification. Hum. Mutat. 2: 309–313.

    Article  CAS  Google Scholar 

  5. Walsh, P.S., Metzger, D.A., and Higuchi, R. 1991 Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Bio Techniques 10: 506–513.

    CAS  Google Scholar 

  6. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989 Molecular Cloning: A Laboratory Manual. 2nd Edn.. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY.

    Google Scholar 

  7. Vogelstein, B. and Gillespie, D. 1979 Preparative and analytic purification of DNA from agarose. Proc. Natl. Acad. Sci. USA 76:615–619.

    Article  CAS  Google Scholar 

  8. Rudi, K. et al. 1997 Rapid, universal method to isolate PCR-ready DNA using magnetic beads. Bio Techniques 22: 506–511.

    CAS  Google Scholar 

  9. Hawkins, T.L. et al. 1994 DNA purification and isolation using a solid-phase. Nucl. Adds Res. 22: 4543–4544.

    Article  CAS  Google Scholar 

  10. Sartor, O. and Zheng, Q. 1997 Determination of CAG repeat length in the androgen-receptor gene using frozen serum. Urol. 49: 301–304.

    Article  CAS  Google Scholar 

  11. Haugland, R.P. 1997 Handbook of Fluorescent Probes and Research Chemicals. 6th Ed. Molecular Probes, Inc., Eugene, OR.

    Google Scholar 

  12. Schoenberg, M.P. et al. 1994 Microsatellite mutation (CAG 24→18) in the androgen receptor gene in human prostate cancer. Biochem. Biophys. Res. Comm. 198: 74–80.

    Article  CAS  Google Scholar 

  13. White, H.W. and Kusukawa, N. Agarose-based system for separation of short tandem repeat loci. Bio Techniques 22: 976–980.

    Article  CAS  Google Scholar 

  14. Becker, A., Reith, A., Napiwotzki, J., and Kadenbach, B. 1996 A quantitative method for determining initial amounts of DNA by polymerase chain reaction cycle titration using digital imaging and a novel DNA stain. Anal. Biochem. 237: 204–207.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. senior staff fellow, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 5A01, Bethesda, MD, 20892

    Shannon C. Dixon

  2. visiting fellow, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 5A01, Bethesda, MD, 20892

    József Horti

  3. predoctoral Fellow, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 5A01, Bethesda, MD, 20892

    Yi Guo

  4. senior investigator, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 5A01, Bethesda, MD, 20892

    Eddie Reed

  5. senior investigator in the Medicine Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 5A01, Bethesda, MD, 20892

    William D. Figg

Authors
  1. Shannon C. Dixon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. József Horti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eddie Reed
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. William D. Figg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dixon, S., Horti, J., Guo, Y. et al. Methods for extracting and amplifying genomic DNA isolated from frozen serum. Nat Biotechnol 16, 91–94 (1998). https://doi.org/10.1038/nbt0198-91

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0198-91

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing