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High Level Expression of the Humanized Monoclonal Antibody CAMPATH-1H in Chinese Hamster Ovary Cells

Bio/Technology volume 9, pages 64–68 (1991)Cite this article

Abstract

We have cloned the light and heavy chain cDNAs for the humanized monoclonal antibody Campath-1H and expressed them in Chinese hamster ovary (CHO) cells using a dihydrofolate reductase (dhfr) amplification procedure. Each cDNA was positioned under control of the strong human β-actin promoter/polyaden-ylation signals and used to evaluate alternative vector design and amplification procedures. By employing a dual selection co-transfection strategy, initial transfor-mants accumulated antibody levels of 0.5 μg/ml after 4 days continuous culture. When subjected to successive rounds of selection in medium containing stepwise increments in methotrexate (MTX), stable cell lines were obtained that secreted up to 200 μg/ml of Campath-1H during the same period. This reflects a productivity of l00 μg/106 cells/day and demonstrates the potential of engineering CHO cells for the production of recombinant antibodies.

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  1. Martin J. Page: Corresponding author.

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  1. Department of Molecular Biology, Wellcome Biotech, Langley Court, Beckenham, Kent, BR3 3BS, UK

    Martin J. Page & Mark A. Sydenham

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  1. Martin J. Page
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Page, M., Sydenham, M. High Level Expression of the Humanized Monoclonal Antibody CAMPATH-1H in Chinese Hamster Ovary Cells. Nat Biotechnol 9, 64–68 (1991). https://doi.org/10.1038/nbt0191-64

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