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Fringe-dependent separation of dorsal and ventral cells in the Drosophila wing

Nature volume 401, pages 476–480 (1999)Cite this article

Abstract

The separation of cells into populations that do not intermix, termed compartments, is a fundamental organizing principle during development1,2,3,4,5. Dorsal–ventral compartmentalization of the Drosophila wing is regulated downstream of the apterous (ap) gene, which encodes a transcription factor that specifies dorsal wing fate6,7,8. fringe (fng) is normally expressed by dorsal cells downstream of ap9; here we show that fng plays a key role in dorsal–ventral compartmentalization. Loss of fng function causes dorsal cells to violate the compartment boundary, and ectopic expression of the Fng protein causes ventral cells to violate the compartment boundary. Fng modulates signalling through the Notch receptor10,11. Notch and its ligands are essential for formation of the dorsal–ventral compartment border, and repositioning the stripe of Notch activation that is normally established there appears to reposition the compartment border. However, activation of Notch does not itself confer either dorsal or ventral cell location, and fng can influence compartmentalization even within regions of ubiquitous Notch activation. Our results indicate that the primary mechanism by which fng establishes a compartment border is by positioning a stripe of Notch activation, but also that fng may exert additional influences on compartmentalization.

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Figure 1: Loss of fng activity causes dorsal cells to violate the compartment boundary.
Figure 2: Ectopic expression of Fng causes ventral cells to violate the compartment border.
Figure 3: Notch signalling is required for D–V compartmentalization.
Figure 4: Influence of ectopic Ser, activated Notch, and activated Notch plus Fng on D–V compartmentalization.

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Acknowledgements

We thank S. Artavanis-Tsakonas, S. Blair, J. de Celis, S. Cohen, D. Doherty, U. Gaul, Y. Hiromi, R. Nusse, G. Struhl, the Developmental Studies Hybridoma Bank and the Bloomington stock center for antibodies and Drosophila stocks. C.R. is a Foundation for Advanced Cancer Studies Fellow of the Life Sciences Research Foundation. This work was supported by a GM NIH grant to K.D.I.

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  1. Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University, Piscataway, 08854, New Jersey, USA

    Cordelia Rauskolb, Trudy Correia & Kenneth D. Irvine

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  1. Cordelia Rauskolb
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  2. Trudy Correia
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  3. Kenneth D. Irvine
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Correspondence to Kenneth D. Irvine.

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Rauskolb, C., Correia, T. & Irvine, K. Fringe-dependent separation of dorsal and ventral cells in the Drosophila wing. Nature 401, 476–480 (1999). https://doi.org/10.1038/46786

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