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Selection system for genes encoding nuclear-targeted proteins

Nature Biotechnology volume 16, pages 1338–1342 (1998)Cite this article

Abstract

Nuclear proteins have essential roles in cell proliferation and differentiation. We have developed a yeast selection system—the nuclear transportation trap (NTT)—to identify genes encoding nuclear transport signals. Both unknown and previously identified nuclear localization signals were identified from a human fetal brain cDNA library. The majority (75%) of the unknown proteins examined were exclusively localized to the nucleus in COS-7 cells. We propose that NTT is an efficient method for isolating cDNAs that encode nuclear targeted proteins that can be applied to the retrieval of novel nuclear proteins and to annotate gene function.

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Figure 1: The nuclear transportation trap system.
Figure 2: NTT assay.
Figure 3: Subcellular distribution analysis of the gene products expressed in COS-7 and yeast cells.

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Acknowledgements

We thank the staff at HRI for supporting our work, and M.B. Swindells for comments regarding the manuscript.

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Author notes

  1. Nobuhide Ueki

    Present address: Pharmaceuticals Discovery Laboratory, Mitsubishi Chemical Corporation, Aoba-ku, Yokohama, 227-8502, Japan

  2. Teruhisa Noguchi

    Present address: TENOX Research Institute, 1-1-1 Uschisaiwai-cho, Chiyoda-du, Tokyo, 100-0011, Japan

Authors and Affiliations

  1. Biological Technology Laboratory, Helix Research Institute , Kisarazu-shi, Chiba, 292-0812, Japan

    Nobuhide Ueki, Tamaki Oda, Maiko Kondo, Kazuhiro Yano, Teruhisa Noguchi & Masa-aki Muramatsu

  2. Department of Biological Cybernetics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-0034, Japan

    Masa-aki Muramatsu

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Correspondence to Nobuhide Ueki.

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Ueki, N., Oda, T., Kondo, M. et al. Selection system for genes encoding nuclear-targeted proteins. Nat Biotechnol 16, 1338–1342 (1998). https://doi.org/10.1038/4315

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