Abstract
We have purified a thermal hysteresis (antifreeze) protein, with up to 100 times the specific activity of fish antifreeze proteins, from the common yellow mealworm beetle, Tenebrio molitor. It is a threonine- and cysteine-rich protein, of relative molecular mass 8,400, composed largely of 12-amino-acid repeats. We estimate that a concentration of roughly 1 mg ml−1 of this protein can account for the 5.5 °C of thermal hysteresis found in Tenebrio larvae (Fig. 1).
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References
Grimstone, A. V., Mullinger, A. M. & Ramsay, J. A. Phil. Trans. R. Soc. Lond. B 253, 343–382 (1968).
Schneppenheim, R. & Theede, H. Comp. Biochem. Physiol. B 67, 561–568 (1980).
Tomchaney, A. P. et al. Biochemistry 21, 716–721 (1982).
Patterson, J. L. & Duman, J. G. J. Exp. Zool. 219, 381–384 (1982).
Horwath, K. L., Easton, C. M., Poggioli, G. J., Myers K. & Schnorr, I. L. Eur. J. Entomol. 93, 419–433 (1996).
Davies, P. L. & Hew, C. L. FASEB J. 4, 2460–2468 (1990).
Wishart, D. S. et al. Comput. Appl. Biosci. 10, 121–132 (1994).
Sönnichsen, F. D., Sykes, B. D. & Davies, P. L. Prot. Sci. 4, 460–471 (1995).
DeVries, A. L. Annu. Rev. Physiol. 45, 245–260 (1983).
Knight, C. A., Cheng, C. C. & DeVries, A. L. Biophys. J. 59, 409–418 (1991).
Wilson, P. W. Cryo-Letters 14, 31–36 (1993).
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Graham, L., Liou, YC., Walker, V. et al. Hyperactive antifreeze protein from beetles. Nature 388, 727–728 (1997). https://doi.org/10.1038/41908
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DOI: https://doi.org/10.1038/41908
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