Abstract
THEaddition of plasma membrane proteins to a growing axon could occur by preferential insertion at the tip (the growth cone), by uniform insertion along the axon, or by insertion at the cell body and bulk flow along the axon. To differentiate between these possibilities we used a defective herpesvirus vector to express an exogenous protein, the lymphocyte transmembrane protein CD8α, in cultured rat hippocampal neurons. The newly synthesized protein first appeared on the axonal surface almost exclusively at the growth cone. Preferential addition at the growth cone was also observed in minor processes (immature dendrites), but not in mature dendrites. Over several hours, CD8α reached a uniform distribution over the entire neuronal surface, presumably by diffusion within the membrane and possibly endocytic recycling. As well as providing materials for axonal growth, the selective addition of membrane vesicles at the growth cone may contribute to the polarized distribution of axonal surface molecules1.
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References
Futerman, A. H., Khanin, R. & Segel, L. A. Nature 362, 119 (1993).
Bray, D. Proc. natn. Acad. Sci. U.S.A. 65, 905–910 (1970).
Koda, L. Y. & Partlow, L. M. J. Neurobiol. 7, 157–172 (1976).
Feldman, E. L., Axelrod, D., Schwartz, M., Heacock, A. M. & Agranoff, B. W. J. Neurobiol. 12, 591–598 (1981).
Pfenninger, K. H. & Maylie-Pfenninger, M.-F. J. Cell Biol. 89, 547–559 (1981).
Griffin, J. W., Price, D. L., Drachman, D. B. & Morris, J. J. Cell Biol. 88, 205–214 (1981).
Small, R. K., Blank, M., Ghez, R. & Pfenninger, K. H. J. Cell Biol. 98, 1434–1443 (1984).
Popov, S., Brown, A. & Poo, M.-M. Science 259, 244–246 (1993).
Roizman, B. & Sears, A. E. in Virology Vol. 2 (eds Fields, B. N. et al. 1795–1841 (Raven, New York, 1990).
Klausner, R. D., Donaldson, J. G. & Lippincott-Schwartz, J. J. Cell Biol. 116, 1071–1080 (1992).
Dotti, C. G., Sullivan, C. A. & Banker, G. A. J. Neurosci. 8, 1454–1468 (1988).
Reinsch, S. S., Mitchison, T. J. & Kirschner, M. J. Cell Biol. 115, 365–379 (1991).
Okabe, S. & Hirokawa, N. J. Cell Biol. 117, 105–120 (1992).
Baas, P. W., Deitch, J. S. & Black, M. M. Proc. natn. Acad. Sci U.S.A. 85, 8335–8339 (1988).
Baas, P. W., Black, M. M. & Banker, G. A. J. Cell Biol. 109, 3085–3094 (1989).
Dotti, C. G. & Simons, K. Cell 62, 62–72 (1990).
Jung, L. J. & Scheller, R. H. Science 251, 1330–1335 (1991).
Mundigl, O. et al. J. Cell Biol. 122, 1207–1221 (1993).
Banker, G. A. & Cowan, M. W. Brain Res. 126, 397–425 (1977).
Goslin, K. & Banker, G. in Culturing Nerve Cells (eds Banker, G. & Goslin, K.) 207–226 (MIT Press, Cambridge, MA, 1991).
Spaete, R. R. & Frenkel, N. Cell 30, 295–304 (1982).
Geller, A. I. & Breakefield, X. O. Science 241, 1667–1669 (1988).
Kaplitt, M. G. et al. Molec. cell. Neurosci. 2, 320–330 (1991).
Littman, D. R., Thomas, Y., Maddon, P. J., Chess, L. & Axel, R. Cell 40, 237–246 (1985).
Kwong, A. D. & Frenkel, N. Virology 142, 421–425 (1985).
DeLuca, N. A., McCarthy, A. M. & Schaffer, P. A. J. Virol. 56, 558–570 (1985).
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Craig, A., Wyborski, R. & Banker, G. Preferential addition of newly synthesized membrane protein at axonal growth cones. Nature 375, 592–594 (1995). https://doi.org/10.1038/375592a0
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DOI: https://doi.org/10.1038/375592a0
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