Abstract
Fertilization of the sea urchin egg stimulates a wave of exocytosis of cortical vesicles1,2, but the mechanism by which fertilization regulates this secretion is not fully understood. We describe here experiments which suggest that polyphosphoinositide metabolism could be a factor in this regulation. We find that the cortical vesicle exocytosis in the egg of Strongylocentrotus purpuratus is preceded by a 40% increase in its content of triphosphoinositide (TPI) and a 22% increase of diphosphoinositide (DPI).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Endo, Y. Expl Cell Res. 25, 383–397 (1961).
Chandler, D. E. & Heuser, J. J. Cell Biol. 83, 91–108 (1979).
Rothschild, L. & Swann, M. M. J. exp. Biol. 29, 469–483 (1952).
Paul, M. & Epel, D. Expl Cell Res. 65, 281–288 (1971).
Jaffe, L. A., Hagiwara, S. & Kado, R. T. Devl Biol. 67, 243–248 (1978).
Schuel, H. Gamete Res. 1, 299–382 (1978).
Eisen, A. thesis, Univ. Pennsylvania (1982).
Steinhardt, R., Zucker, R. & Schatten, G. Devl Biol. 58, 185–196 (1977).
Zucker, R. S. & Steinhardt, R. A. Biochim. biophys. Acta 541, 459–466 (1978).
Hamaguchi, Y. & Hiramoto, Y. Expl Cell Res. 134, 171–179 (1981).
Baker, P. F. & Whitaker, M. J. Nature 276, 513–515 (1978).
Steinhardt, R. A. & Alderton, J. M. Nature 295, 154–155 (1982).
Picard, A. & Dorée, M. Devl Growth Different. 24, 155–162 (1982).
Whitaker, M. J. & Baker, P. F. Proc. R. Soc. B218, 397–413 (1983).
Billah, M. M. & Lapetina, E. G. J. biol. Chem. 257, 12705–12708 (1982).
Weiss, S. J., McKiney, J. S. & Putney, J. W. Biochem. J. 206, 555–560 (1982).
Agranoff, B. W., Murthy, P. & Seguin, E. B. J. biol. Chem. 258, 2076–2078 (1983).
Perret, B. P., Plantavid, M., Chap, H. & Douste-Blazy, L. Biochem. biophys. Res. Commun. 110, 660–667 (1983).
Kinsey, W. H., Decker, G. L. & Lennarz, W. J. J. Cell Biol. 87, 248–254 (1980).
Schmell, E. & Lennarz, W. J. Biochemistry 13, 4114–4121 (1974).
Chambers, E. L. & Whiteley, A. H. J. cell. Physiol. 68, 289–308 (1966).
Streb, H., Irvine, R. F., Berridge, M. J. & Schulz, I. Nature 306, 67–69 (1983).
Papahadjopoulos, D., Poste, G. & Schaeffer, B. E. Biochim. biophys. Acta 323, 23–442 (1973).
Downes, P. & Michell, R. H. Cell Calcium 3, 467–502 (1982).
Sheetz, M. P., Febbroriello, P. & Koppel, D. E. Nature 296, 91–93 (1982).
Epel, D. Curr. Topics dev. Biol. 12, 185–246 (1978).
Schacht, J. J. Lipid Res. 19, 1063–1067 (1978).
Ames, B. N. Meth. Enzym. 8, 115–118 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Turner, P., Sheetz, M. & Jaffe, L. Fertilization increases the polyphosphoinositide content of sea urchin eggs. Nature 310, 414–415 (1984). https://doi.org/10.1038/310414a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/310414a0
This article is cited by
-
New insights into negative effects of lithium on sea urchin Paracentrotus lividus embryos
Scientific Reports (2016)
-
Signal transduction in mammalian oocytes during fertilization
Cell and Tissue Research (2016)
-
Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta
BMC Developmental Biology (2008)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
