Abstract
The increase in cytosolic [Ca2+] induced by Ca-mobilizing hormones in liver is mainly due to release of Ca from intracellular stores1–7. For Ca to be released from internal sites a messenger must be formed at the plasma membrane which diffuses into the cytosol to signal Ca release from the intracellular organelles2,3,7,8. One of the first actions of these hormones is to cause breakdown of the polyphosphoinositides to form soluble inositol phosphates9–11. Some evidence for the idea that these substances could be the second messenger10 has been obtained in pancreatic acinar cells12. Here we have found that hormone activation of hepatocytes causes rapid breakdown of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] to form inositol trisphosphate (InsP3). When applied to permeabilized hepatocytes, InsP3 releases Ca from non-mitochondrial ATP-dependent pools. This suggests that InsP3 could be the messenger linking Ca-mobilizing receptor activation to intracellular Ca release in liver.
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Burgess, G., Godfrey, P., McKinney, J. et al. The second messenger linking receptor activation to internal Ca release in liver. Nature 309, 63–66 (1984). https://doi.org/10.1038/309063a0
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DOI: https://doi.org/10.1038/309063a0
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