Abstract
Lymphocytes are known to interact directly with other cells in vivo1,2 and in vitro3–5, and have recently been shown to transfer the lysosomal enzyme, β-glucuronidase, to fibroblasts from patients with an inherited deficiency of the enzyme6. This process requires cell–cell contact, is unaffected by inhibitors of ‘classical’ receptor-mediated endocytosis and is abolished by inhibitors of protein synthesis7. Although it is not yet known to what extent the transfer of enzymes by direct cellular interaction is a general phenomenon, a similar mechanism could possibly be involved in the transfer of other lysosomal enzymes in vivo8–10 and in the exchange of protein in vitro11. We show here that the direct transfer of enzymes from lymphocytes to fibroblasts is restricted to only certain lysosomal enzymes.
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Olsen, I., Muir, H., Smith, R. et al. Direct enzyme transfer from lymphocytes is specific. Nature 306, 75–77 (1983). https://doi.org/10.1038/306075a0
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DOI: https://doi.org/10.1038/306075a0
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