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Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29

Nature volume 301, pages 722–725 (1983)Cite this article

Abstract

Avian myelocytomatosis virus MC29 is a replication-defective acute leukaemia virus which induces a variety of tumours in chickens including sarcomas, renal and hepatic carcinomas, and myelocytomatosis1. The oncogenic potential of the virus is mediated by the gene v-myc, acquired from sequences (c-myc) present in normal uninfected chicken DNA2. Sequences closely related to chicken c-myc have been highly conserved throughout evolution, from Drosophila to vertebrates3,4. The hypothesis that c-myc may be involved in neoplastic transformation has been strengthened by the finding that B-cell lymphomas induced in chickens by avian leukosis virus (ALV) are often associated with increased expression of c-myc resulting from integration of the ALV provirus adjacent to the c-myc gene5,6. More recently, it has been demonstrated that the malignant human cell line HL-60, derived from the peripheral blood leukocytes of a patient with acute promyelocytic leukaemia, expresses elevated levels of myc-related mRNA7 associated with an amplification of the c-myc gene8,9. To explore the relationship of the human cellular myc gene with the corresponding viral oncogene from MC29, and to provide a framework for the analysis of the mechanism and significance of c-myc amplification in human tumours, we have isolated and determined the nucleotide sequence of a genomic done prepared from a normal human library which contains all domains sharing homology with v-myc.

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Authors and Affiliations

  1. Department of Molecular Biology, Genentech Inc., 460 Pt San Bruno Boulevard, South San Francisco, California, 94080, USA

    Wendy W. Colby, Ellson Y. Chen, Douglas H. Smith & Arthur D. Levinson

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  1. Wendy W. Colby
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  2. Ellson Y. Chen
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  4. Arthur D. Levinson
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Colby, W., Chen, E., Smith, D. et al. Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29. Nature 301, 722–725 (1983). https://doi.org/10.1038/301722a0

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