Abstract
Most of the mRNA sequences coding for α and β tubulin in embryonic chick brain have been determined by sequencing of cloned cDNA copies of these mRNAs. From a 1,682-base pair cDNA sequence we have deduced the entire protein sequence for β tubulin. For α tubulin, all but about 38 N-terminal amino acids have been deduced from the cDNA sequence. Although tyrosine has previously been shown to be post-translationally added to the C-terminus of α tubulin by a specific ligase, we conclude that the primary post-translational event must be the removal, not the addition, of tyrosine because a terminal tyrosine is encoded by the mRNA.
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References
Roberts, K. & Hyams, J. S. (eds) Microtubules (Academic, New York, 1979).
Kirschner, M. W. Int. Rev. Cytol. 54, 1–71 (1979).
Cleveland, D. W. et al. Cell 20, 95–105 (1980).
Sanchez, F., Natzle, J., Cleveland, D. W., Kirschner, M. W. & McCarthy, B. J. Cell 22, 845–854 (1980).
Luduena, R. F. & Woodward, D. O. Proc. natn. Acad. Sci. U.S.A. 70, 3594–3598 (1973).
Lu, R. C. & Elzinga, M. Biochim. biophys. Acta 537, 320–328 (1978).
Ponstingl, H., Little, M., Krauhs, E. & Kempf, T. Nature 282, 423–424 (1979).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Feit, H., Slusarek, L. & Shelanski, M. L. Proc. natn. Acad. Sci. U.S.A. 68, 2028–2032 (1971).
Cleveland, D. W., Kirschner, M. W. & Cowan, N. J. Cell 15, 1021–1031 (1978).
Arce, C. A., Rodriguez, J. A., Barra, H. S. & Caputto, R. Eur. J. Biochem. 59, 145–149 (1975).
Argarana, C. E., Arce, C., Barra, H. S. & Caputto, R. Archs Biochem. Biophys. 180, 264–268 (1977).
Raybin, D. & Flavin, M. Biochem. biophys. Res. Commun. 65, 1088–1095 (1975).
Raybin, D. & Flavin, M. Biochemistry 16, 2189–2194 (1977).
Rodriguez, J. A. & Borisy, G. G. J. Cell Biol. 75, 296a (1978).
McReynolds, L. et al. Nature 273, 723–728 (1978).
Czernilofsky, A. P., Levinson, A. D., Varmus, H. E. & Bishop, J. M. Nature 287, 198–203 (1980).
Ganier, J., Osguthorpe, D. J. & Robson, B. Molec. Biol 120, 97–120 (1978).
Chou, P. Y. & Fasman, G. D. Biochemistry 13, 222–245 (1974).
Krauhs, E., Little, M., Kempf, T. & Ponstingl, H. Eur. J. Biochem. 22, 285 (1980).
Collins, J. H. & Elzinga, M. J. biol. Chem. 250, 5906–5920 (1975).
Lu, R. C. & Elzinga, M. Biochemistry 16, 5801–5806 (1977).
Vandekerchove, J. & Weber, K. Proc. natn. Acad. Sci. U.S.A. 75, 1106–1110 (1978).
Vandekerchove, J. & Weber, K. Nature 276, 720–721 (1978).
Gallwitz, D. & Sures, I. Proc. natn. Acad. Sci. U.S.A. 77, 2546–2550 (1980).
Ng, R. & Abelson, J. Proc. natn. Acad. Sci. U.S.A. 77, 3912–3916 (1980).
MacDonald, R. J. et al. Nature 287, 117–122 (1980).
Hobart, P., Crawford, R., Shen, L.-P., Pictet, R. & Rutter, W. J. Nature 288, 137–141 (1980).
Kemphues, K. J., Raff, R. A., Kaufman, T. C. & Raff, E. C. Proc. natn. Acad. Sci. U.S.A. 76, 3991–3995 (1979).
Kemphues, K. J., Raff, E. C., Raff, R. A. & Kaufman, T. C. Cell 21, 445–454 (1980).
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Valenzuela, P., Quiroga, M., Zaldivar, J. et al. Nucleotide and corresponding amino acid sequences encoded by α and β tubulin mRNAs. Nature 289, 650–655 (1981). https://doi.org/10.1038/289650a0
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DOI: https://doi.org/10.1038/289650a0
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