Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Induction of macrophage production and proliferation by a purified colony stimulating factor

Nature volume 274, pages 168–170 (1978)Cite this article

Abstract

COLONY STIMULATING FACTORS (CSFs) stimulate isolated haemopoietic progenitor cells to differentiate and form granulocyte and/or macrophage colonies in vitro1,2. There seem to be at least two distinct subclasses of CSF, those stimulating macrophage colony formation and those stimulating granulocytic colony formation. Purified CSF from mouse L cell-conditioned medium is a 70,000 molecular weight glycoprotein, comprising two disulphide-bonded 35,000 MW subunits3. At concentrations as low as 10−12 M it stimulates macrophage colony formation by murine bone marrow cells. It has similar physicochemical properties to, and shared antigenic determinants with, the CSF from human urine3–6. This type of CSF has been shown6 to share identity with the macrophage growth factor of Virolainen and Defendi7 that stimulates proliferation of peritoneal exudate macrophages. The purified CSF was also active6 in stimulating macrophage colony formation by peritoneal exudate cells in the agar culture system of Lin and Stewart8. We now report that this same kind of CSF is the molecule responsible for the proliferation of mononuclear phagocytes from several haemopoietic and nonhaemopoietic tissues.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Pluznik, D. H. & Sachs, L. J. cell. Physiol. 66, 319–324 (1965).

    Article  CAS  Google Scholar 

  2. Bradley, T. R. & Metcalf, D. Aust. J. exp. Biol. med. Sci. 44, 287–299 (1966).

    Article  CAS  Google Scholar 

  3. Stanley, E. R. & Heard, P. M. J. biol. Chem. 252, 4305–4312 (1977).

    CAS  PubMed  Google Scholar 

  4. Stanley, E. R. & Metcalf, D. Aust. J. exp. Biol. med. Sci. 47, 467–483 (1969).

    Article  CAS  Google Scholar 

  5. Stanley, E. R., Hansen, G., Woodcock, J. & Metcalf, D. Fedn Proc. 34, 2272–2278 (1975).

    CAS  Google Scholar 

  6. Stanley, E. R., Cifone, M., Heard, P. M. & Defendi, V. J. exp. Med. 143, 631–647 (1976).

    Article  CAS  Google Scholar 

  7. Virolainen, M. & Defendi, V. Wistar Inst. Symp. Monogr. 7, 67–85 (1967).

    CAS  PubMed  Google Scholar 

  8. Lin, H.-S. & Stewart, C. C. J. cell. Physiol. 83, 369–378 (1974).

    Article  CAS  Google Scholar 

  9. Worton, R. G., McCulloch, E. A. & Till, J. E. J. cell. Physiol. 74, 171–182 (1969).

    Article  CAS  Google Scholar 

  10. Till, J. E., Whitmore, G. F. & Gulyas, S. Biochim. biophys. Acta 72, 277–289 (1963).

    Article  CAS  Google Scholar 

  11. Stanley, E. R., Metcalf, D., Maritz, J. S. & Yeo, G. F. J. Lab. clin. Med. 79, 657–668 (1972).

    CAS  PubMed  Google Scholar 

  12. Metcalf, D. J. cell. Physiol. 76, 89–99 (1970).

    Article  CAS  Google Scholar 

  13. Lin, H.-S., Kuhn, C. & Kuo, T. T. J. exp. Med. 142, 877–886 (1975).

    Article  CAS  Google Scholar 

  14. Lin, H.-S. Blood 49, 593–598 (1977).

    CAS  PubMed  Google Scholar 

  15. Berg, T. & Boman, D. Biochim. biophys. Acta 321, 585–596 (1973).

    Article  CAS  Google Scholar 

  16. Chen, D.-M., Lin, H.-S., Stahl, P. & Stanley, E. R. (in preparation).

  17. Soderland, S. C. & Naum, Y. Nature 245, 150–152 (1973).

    Article  ADS  CAS  Google Scholar 

  18. Naum, Y. Cytobios 14, 211–220 (1975).

    Google Scholar 

  19. Bennett, B. J. exp. Med. 48, 165–181 (1966).

    CAS  Google Scholar 

  20. Adolphe, M., Fontagne, J., Pelletier, M. & Giroud, J. P. Nature 253, 637 (1975).

    Article  ADS  CAS  Google Scholar 

  21. Wynne, K. M., Spector, W. G. & Willoughby, D. A. Nature 253, 636–637 (1957).

    Article  ADS  Google Scholar 

  22. Bradley, T. R., Metcalf, D., Sumner, M. & Stanley, R. In Vitro 4, 22–35 (1969).

    Article  Google Scholar 

  23. Chu, J. & Lin, H.-S. J. reticuloendothel. Soc. 20, 299–305 (1976).

    CAS  PubMed  Google Scholar 

  24. Mac Vittie, T. J. & McCarthy, K. F. J. cell. Physiol. 92, 203–208 (1977).

    Article  CAS  Google Scholar 

  25. Stanley, E. R. J. Supramolec. Struct. Suppl. 2, 175 (1978).

  26. Guilbert, L. J. & Iscove, N. N. Nature 263, 594–595 (1976).

    Article  ADS  CAS  Google Scholar 

  27. VanFurth, R. et al. Bull. W.H.O. 46, 845–852 (1972).

    CAS  Google Scholar 

  28. Aschoff, L. Ergebn. Inn. Med. Kunderheilk. 26, 1–118 (1924).

    Google Scholar 

  29. Kurland, J. & Moore, M. A. S. Expl Hemat. 5, 357–373 (1977).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Microbiology and Immunology, and Cell Biology, Albert Einstein College of Medicine, Bronx, New York, 10461

    E. R. STANLEY

  2. Section of Cancer Biology, Divison of Radiation Oncology, Mallinkrodt Institute of Radiology, Washington University School of Medicine, 510 South Kingshighway, St Louis, Missouri, 63110

    D.-M. CHEN & H.-S. LIN

Authors
  1. E. R. STANLEY
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D.-M. CHEN
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H.-S. LIN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

STANLEY, E., CHEN, DM. & LIN, HS. Induction of macrophage production and proliferation by a purified colony stimulating factor. Nature 274, 168–170 (1978). https://doi.org/10.1038/274168a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/274168a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing