Abstract
CULTURE media for mammalian cells usually require supplementation with serum to supply as yet undefined needs. Because the requirements are likely to be multiple, it is difficult to distinguish biologically between the effects of nonspecific ‘nutritional’ factors in serum and those of specific regulatory factors. Partial replacement of serum in these systems by chemically defined substances supplying the nonspecific needs would therefore represent a significant advance towards the definition of such specific factors. We have examined the role of several serum components in cultures of freshly explanted haemopoietic cells with this goal in mind.
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GUILBERT, L., ISCOVE, N. Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoitec cell cultures. Nature 263, 594–595 (1976). https://doi.org/10.1038/263594a0
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DOI: https://doi.org/10.1038/263594a0
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