Abstract
MOST fibrous proteins do not crystallise. An artful way to promote protein crystallisation is to modify the structure by proteolytic attack. For example, papain cleavage of γ-globulin yields the homogeneous Fc fragment which readily crystallises1. Following a similar approach, we have crystallised fibrinogen.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cohen, S., and Porter, R. R., Adv. Immun., 4, 287 (1964).
Cohen, C., Slayter, K., Goldstein, L., Kucera, J., and Hall, G., J. molec. Biol., 22, 385 (1966).
Tooney, N. M., and Cohen, C., Nature, 237, 23 (1972).
Weber, K., and Osborn, M., J. biol. Chem., 244, 4406 (1969).
Henschen, A., Arkiv. Kemi., 22, 1 (1964).
McKee, P. A., Rogers, L. A., Marler, E., and Hill, R. L., Arch. Biochem. Biophys., 116, 271 (1966).
Scheraga, H. A., and Laskowski, M., Adv. Protein Chem., 12, 1 (1957).
Hodge, A. J., and Schmitt, F. O., Proc. natn. Acad. Sci. U.S.A., 46, 186 (1960).
Cohen, C., Szent-Gyorgyi, A. G., and Kendrick-Jones, J., J. molec. Biol., 56, 223 (1971).
Cohen, C., Lowey, S., Harrison, R. G., Kendrick-Jones, J., and Szent-Gyorgyi, A. G., J. molec. Biol., 47, 605 (1970).
Squire, J. M., J. molec. Biol., 77, 291 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
COHEN, C., TOONEY, N. Crystallisation of a modified fibrinogen. Nature 251, 659–660 (1974). https://doi.org/10.1038/251659a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/251659a0
This article is cited by
-
Is myelin basic protein crystallizable?
Neurochemical Research (1992)
-
A new myosin fragment: visualization of the regulatory domain
Nature (1984)
-
A model from electron microscopy for the molecular structure of fibrinogen and fibrin
Nature (1981)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.