Abstract
RELEASE of catecholamines from the chromaffin granules of the adrenal medulla and from sympathetic nerve endings is accompanied by release of chromogranins, specific soluble proteins contained within vesicles containing noradrenaline. Since protein (chromogranin) release is dependent on calcium and is stoichiometrically related to the amount of catechplamine released, release is thought to be by exocytosis1–3. We know no report of protein released at cholinergic synapses, but biochemical studies of isolated cholinergic vesicles have revealed that many different protein components are contained within these vesicles4. We report here evidence indicating that proteins are released from nerve terminals of the neuromuscular junction.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Smith, A. D., de Potter, W. P., Moerman, E. J., and de Schaepdryver, A. F., Tissue and Cell, 2, 547 (1970).
Geffen, L. B., Livett, B. G., and Rush, R. A., in New Aspects of Storage and Release Mechanisms of Catecholamines, International Bayer Symposium, 2, 58 (Springer, Heidelburg, 1970).
Gewirtz, G. P., and Kopin, I. J., Nature, 227, 406 (1970).
Potter, L. T., Neurosciences Res. Prog. Bull., 8, 384 (1970).
Liley, A. W., J. Physiol., 132, 650 (1956).
Lowry, O. H., Rosebrough, N. H., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Davis, B. J., Ann. N Y Acad. Sci., 121, 404 (1964).
Chrambach, A., Reisfeld, R. A., Wyckoff, M., and Zaccari, J., Anal. Biochem., 20, 150 (1967).
Zeirler, K. L., Amer. J. Physiol., 193, 534 (1958).
Dawson, D. M., Biochim. Biophys. Acta, 113, 144 (1966).
Hubbard, J. I., Biophys. Mol. Biol., 21, 33 (1970).
Szilagyi, P. I. A., Schmidt, D. E., and Green, J. P., Anal. Chem., 40, 2009 (1968).
Geffen, L. B., and Livett, B. G., Physiol. Rev., 51, 98 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MUSICK, J., HUBBARD, J. Biological Sciences: Release of Protein from Mouse Motor Nerve Terminals. Nature 237, 279–281 (1972). https://doi.org/10.1038/237279a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/237279a0
This article is cited by
-
Release of acetylcholinesterase from rat hemidiaphragm preparations stimulated through the phrenic nerve
Nature (1978)
-
In Vitro studies of skeletal muscle membranes
The Journal of Membrane Biology (1977)
-
A cholinergic modulator
Experientia (1977)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
