Abstract
CELLS derived from the mouse neuroblastoma (C1300) grown in culture are predominantly round and highly refractile. A small number of the cells are flatter and possess one or more extensions (“neurites”) which may be as long as 3 mm1–3. The proportion of these morphologically differentiated cells increases when the medium lacks serum4 or when 5-bromo-deoxyuridine is added5. The cells also contain enzymes of neural function2,6 and are capable of generating action potentials in vitro7,8. We now show that differentiated characteris-tics of neuroblastoma cells can be induced by N6,O2′-dibutyryl adenosine 3′ : 5′-monophosphate (dibutyryl-cyclicAMP).
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FURMANSKI, P., SILVERMAN, D. & LUBIN, M. Expression of Differentiated Functions in Mouse Neuroblastoma mediated by Dibutyryl-cyclic Adenosine Monophosphate. Nature 233, 413–415 (1971). https://doi.org/10.1038/233413a0
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DOI: https://doi.org/10.1038/233413a0
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