Abstract
SHORTLY after the development of dextran gels as packing material for size separation of solutes by gel chromatography (gel filtration)1, the sorption properties for low molecular weight aromatic substances were observed2. In spite of numerous investigations, some of which compared molecular structure with degree of retardation, and some of which exploited the side effects of gel chromatography for actual separation problems3, no interpretation was given for these rather strong interactions. Aromatic and heterocyclic substances are sometimes retarded during liquid chromatography on various sorbents such as charcoal, cellulose, silica, alumina oxide and polyacrylamide3, but the effects are much less pronounced than in the case of ‘Sephadex’ dextran gels. The sorption properties of ‘Sephadex’ gels should be divided into two classes: (a) the “mass action” and (b) special “binding” forces between solutes with π-electrons and the ‘Sephadex’ matrix. It is the nature of this specific binding that we wish to describe.
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References
Porath, J., and Flodin, P., Nature, 183, 1657 (1959).
Gelotte, B., J. Chromatog., 3, 330 (1960).
Determann, H., Gel Chromatography (Springer, Berlin, Heidelberg, New York, 1967).
Gelotte, B., and Porath, J., in Chromatography (edit. by Heftman) (Reinhold Pub. Corp., New York, 1967).
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DETERMANN, H., WALTER, I. Source of Aromatic Affinity to ‘Sephadex’ Dextran Gels. Nature 219, 604–605 (1968). https://doi.org/10.1038/219604a0
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DOI: https://doi.org/10.1038/219604a0
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