Abstract
Labelled inhibitors have been used to study the activity and properties of a chymotrypsin-like enzyme in rat mast cells. In conjunction with light microscopy (this article) and electron microscopy (second article, page 1202), the method yields estimates of the numbers and locations of the enzyme molecules.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gomori, G., J. Histochem. Cytochem., 1, 469 (1953).
Benditt, E. P., and Arase, M., J. Histochem. Cytochem., 6, 431 (1958).
Benditt, E. P., and Arase, M., J. Exp. Med., 110, 451 (1959).
Lagunoff, D., and Benditt, E. P., Nature, 192, 1198 (1961).
Lagunoff, D., and Benditt, E. P., Ann. N.Y. Acad. Sci., 103, 185 (1963).
Glenner, G. G., and Cohen, L. A., Nature, 185, 846 (1960).
Glenner, G. G., Hopsu, V. K., and Cohen, L. A., J. Histochem. Cytochem., 10, 109 (1962).
Lagunoff, D., Benditt, E. P., and Watts, R. M., J. Histochem. Cytochem., 10, 672 (1962).
Ende, N., Katayama, Y., and Auditore, J. V., Nature, 201, 1197 (1964).
Spector, W. G., and Willoughby, D. A., J. Path. Bact., 79, 21 (1960).
Ungar, G. T., Tamura, T., Isole, J. B., and Kobrin, S., J. Exp. Med., 113 359 (1961).
Pastan, I., and Almquist, S., Endocrinology, 78, 361 (1966).
Ostrowski, K., and Barnard, E. A., Exp. Cell Res., 25, 465 (1961).
Thursh, D. R., and Benditt, E. P., J. Histochem. Cytochem., 9, 616 (1961).
Ostrowski, K., Barnard, E. A., Darżynkiewicz, Z., and Rymaszewska, D., Exp. Cell Res., 36, 43 (1964).
Darżynkiewicz, Z., Rogers, A. W., and Barnard, E. A., J. Histochem. Cytochem., 14, 379 (1966).
Darżynkiewicz, Z., Rogers, A. W., Barnard, E. A., Wang, D. H., and Werkheiser, W. C., Science, 151, 1528 (1966).
Rogers, A. W., Darżynkiewicz, Z., Barnard, E. A., and Salpeter, M., Nature, 210, 1003 (1966).
Cohen, J. A., and Oosterbaan, R. A., in Handbuch der Exp. Pharmakologie, 15, 299 (edit. by Koelle, G. B.) (Springer-Verlag, Berlin, 1963).
Levi, H., Rogers, A. W., Bentzon, M. W., and Nielsen, A., Mat. Fys. Medd. Dan. Vid. Selsk., 33, 11 (1963).
Darżynkiewicz, Z., Rogers, A. W., and Barnard, E. A., J. Histochem. Cytochem., 14, 915 (1966).
Jandorf, H. O., Schaffer, N. K., Egan, R., and Summerson, W. H., Disc. Faraday Soc., 20, 134 (1955).
Fong, C. T. O., Becker, R. R., and Louie, D. D., Biochim. Biophys. Acta, 90, 619 (1964).
Greenberg, H., and Nachmansohn, D., Biochem. Biophys. Res. Commun., 7, 186 (1962).
Schoellman, G., and Shaw, E., Biochemistry, 2, 252 (1963).
Shaw, E., Mares-Guia, M., and Cohen, W., Biochemistry, 4, 2219 (1965).
Myers, D. K., in The Enzymes, 4 (edit. by Bover, P. D., Lardy, H., and Myrbach, K.) (Academic Press, New York:1960).
Hummel, B. C. W., Canad. J. Biochem. Physiol., 37, 1393 (1959).
Huang, H. T., and Niemann, C., J. Amer. Chem. Soc., 74, 101 (1952).
Geratz, J. D., Experientia, 21, 699 (1965).
Ottoson, R., Kahn, K., and Glick, D., Exp. Cell Res., 14, 567 (1958).
Lagunoff, D., Phillips, M. T., Iseri, O. A., and Benditt, E. P., Lab. Invest., 13, 1331 (1964).
Kawiak, J., Darżynkiewicz, Z., and Barnard, E. A., J. Cell Biol., 31, 58A (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DARŻYNKIEWICZ, Z., BARNARD, E. Specific Proteases of the Rat Mast Cell. Nature 213, 1198–1202 (1967). https://doi.org/10.1038/2131198a0
Issue Date:
DOI: https://doi.org/10.1038/2131198a0
This article is cited by
-
Compound 48/80-induced secretion of histamine from rat peritoneal mast cells depends on a tryptase controlled step also leading to chymase activity
Agents and Actions (1990)
-
Hyposmotic swelling leads to the expression of trypsin-like activity by rat peritoneal fluid mast cells
Agents and Actions (1987)
-
Existence of an α-chymotrypsin-like immunoreactivity in bovine mast cells
Experientia (1982)
-
Enhancing effect of heparin on aprotinin activity
Experientia (1981)
-
The labelled inhibitor method in cytoenzymology
The Histochemical Journal (1972)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.