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Antimicrobial and antiviral activities of an actinomycete (Streptomyces sp.) isolated from a Brazilian tropical forest soil

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Abstract

A promising producer of bioactive compounds isolated from a Brazilian tropical soil was tested for its range of antimicrobial activities. Strain 606, classified as Streptomyces sp., could not be identified up to species level, suggesting a possible new taxon. The supernatant and 10 extracts and fractions, obtained by extraction and chromatographic techniques, presented antimicrobial activity using antibiograms. The methanolic fraction was highly active against pathogenic bacteria, phytopathogenic fungi and the human pathogenic yeast Candida albicans. It also possessed high antiviral activity inhibiting the propagation of an acyclovir-resistant herpes simplex virus type 1 strain on HEp-2 cells at non-cytotoxic concentration. The strong cytotoxic effect suggests an antitumour action.

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References

  • Anderson, A.L. & Wellington, E. 2001 The taxonomy of Streptomyces and related genera. International Journal of Systematic and Evolutionary Microbiology 51, 797–814.

    CAS  Google Scholar 

  • Barry, A.L. & Thornsberry, C. 1985 Susceptibility testes: diffusion test procedure. In Manual of Clinical Microbiology, 4th edn., eds. Ballows, E.A., Hawsler,W.J. Jr. & Shadomy, H.I. pp. 978–987. Washington DC: American Society of Microbiology. ISBN 0-914826-65-4.

    Google Scholar 

  • Becker, B., Lechevalier, M.P., Gordon, R.E. & Lechevalier, H.R. 1964 Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolisates. Applied Microbiology 12, 12421–12423.

    Google Scholar 

  • Brito, M.A.V.P., Lagrota, M.H.C. & Machado, R.D. 1981 Emprego de microténica na triagem de substâncias antivirais. Revista de Microbiologia 1, 65–69.

    Google Scholar 

  • Bull, A.T., Goodfellow, M. & Slatter, J.H., 1992 Biodiversity as a source of innovation in biotechnology. Annual Review of Microbiology 46, 152–219.

    Article  Google Scholar 

  • Chen, M., Lucia, H.L. & Swing, G.D. 1988 Efficacy of S26308 against guinea pig cytomagalovirus infection. Antimicrobial Agents and Chemotherapy 32, 678–683.

    CAS  Google Scholar 

  • Chino, M., Nishimura, K., Umekita, M., Hayashi, C., Yumazaki, T., Tsuchida, T., Sawa, T., Hamada, M. & Takeuchi, T. 1996 Heliquinomycin, a new inhibitor of DNA-helicase, produced by Streptomyces sp. MJ929-SF2. I — Taxonomy, production, isolation, physico-chemical properties and biological activities. Journal of Antibiotics 49, 752–757.

    CAS  Google Scholar 

  • Chun, J. & Manfio, G.P. 1994 Program X for Bacterial Systematics. Version 1.0. Copyright 1993–1994 by Jongsik Chun. Department of Microbiology, The Medical School, Framlington Place, Newcastle-upon-Tyne NE24HH, UK.

    Google Scholar 

  • Cross, T. 1989 Growth and examination of Actinomycetes — some guidelines. In Bergey's Manual of Systematic Bacteriology, Vol. 4, eds. Williams, S.T., Sharpe, M.E. & Holt, J.G. pp. 2340–2343. Baltimore: Williams and Wilkins. ISBN 0-683-09061-5.

    Google Scholar 

  • Demain, A.L. 1995 Why do microorganisms produce antimicrobials? In Fifty Years of Antimicrobials: Past, Prospective and Future Trends — Symposium 53, eds. Hunter, P.A., Darby, G.K. & Russell, N.J. pp. 205–228. Society of General Microbiology, Cambridge University Press.

  • Gonzáles, I., Niebla, A., Lemus, M., Gonzáles, L., Otero, I., Iznaga, Y., Pérez, M.E. & Vallin, C., 1999 Ecological approach of macrolide-lincosamides-streptogramin producing Actinomyces from Cuban soils. Letters in Applied Microbiology 29, 147–150.

    Article  Google Scholar 

  • Goodfellow, M. Williams, S.T. & Mordarski, M. 1988 Actinomycetes in Biotechnology, eds. Goodfellow, M., Williams, S.T. & Mordarski, M. London: Academic Press. ISBN 0-12-289673-4.

    Google Scholar 

  • Hopkins, D.W., Macnaughton, S.J. & O'Donnell, A.G. 1991 A dispersion and differential centrifugation technique for representatively sampling microorganisms from soil. Soil Biology and Biochemistry 23, 217–225.

    Article  Google Scholar 

  • Labeda, D.P. 1992 DNA-DNA hybridization in the Systematics of Streptomyces. Gene 115, 249–253.

    Article  CAS  Google Scholar 

  • Miranda, M.M.F.S., Almeida, A.P., Costa, S.S., Santos, M.G.M., Lagrota, M.H.C. & Wigg, M.D. 1997 In vitro activity of extracts of Persea americana leaves on acyclovir resistant and phosphonoacetic resistant herpes simplex virus. Phytomedicine 4, 347–352.

    CAS  Google Scholar 

  • Moore-Landecker, E. 1996 Fungi in pathological relationships of agricultural importance. In Fundamental of Fungi, 4th edn., eds. Snavely S.R. et al., pp. 404–452. New Jersey: Prentice-Hall Inc. ISBN 0-13-376864-3.

    Google Scholar 

  • Nishimura, T., Toku, K. & Fukuyasy, H. 1977 Antiviral compounds. XII. Antiviral activity of aminohydrazones of alkoxylphenyl-substituted carbonyl compounds against influenza virus in eggs and mice. Kitasato Archives of Medicine 50, 39–46.

    CAS  Google Scholar 

  • Nolan, R.D. & Cross, T. 1988 Isolation and screening of actinomycetes. In Actinomycetes in Biotechnology, eds. Goodfellow, M., Williams, S.T. & Mordarski, M.M. London: Academic Press. ISBN 0-12-289673-4.

    Google Scholar 

  • Prauser, H., Schuman, P., Rainey, F.A., Kroppenstedt, R.M. & Stackebrandt, E. 1997 Terracoccus luteus gen. Nov., sp. Nov., an LL-diaminopimelic acid-containing coccoid actinomycete from soil. International Journal of Systematic Bacteriology 47, 1218–1224.

    CAS  Google Scholar 

  • Reed, L.J. & Muench, H. 1938 A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27, 493–497.

    Google Scholar 

  • Sanglier, J.J., Wellington, E.M.H., Behal, V., Fiedler, H.P., Ellouz Ghorbel, R., Finance, C., Hacene, M., Kamoun, A., Kelly, C., Mercer, D.K., Prinzis, S. & Trigo, C. 1993 Novel bioactive compounds from actinomycetes. Research on Microbiology 144, 661–663.

    Article  CAS  Google Scholar 

  • Semêdo, L.T.A. 1997 Atividade antimicrobiana e celulolítica de actinomicetos isolados de solos brasileiros. Ms. thesis, UFRJ, Rio de Janeiro, Brasil.

    Google Scholar 

  • Semêdo, L.T.A., Linhares, A.A., Gomes, R.C., Manfio, G.P., Alviano, C.S. Linhares, L.F. & Coelho, R.R.R. 2001 Isolation and characterization of actinomycetes from Brazilian tropical soils. Microbiological Research 155, 291–299.

    Google Scholar 

  • Singh, S.K., Gurusiddaiah, S. & Whalen, J.W. 1985 Treponemyn, a nitrile antibiotic active against Treponema hyodysenteriae. Antimicrobial Agents and Chemotherapy 27, 329–345.

    Google Scholar 

  • Tsvetanova, B.C. & Price, N.P.J. 2001 Liquid chromatography-electrospray mass spectrometry of tunicamycin-type antibiotics. Analytical Biochemistry 289, 147–156.

    Article  CAS  Google Scholar 

  • Williams, S.T. Goodfellow, M., Alderson, G., Wellington, E.M.H., Sneath, P.H. & Sackin, M.J. 1983a Numerical classification of Streptomyces and related genera. Journal of General Microbiology 129, 1743–1813.

    CAS  Google Scholar 

  • Williams, S.T., Goodfellow, M., Wellington, E.M.H., Vickers, J.C., Alderson, G., Sneath, P.H.A., Sackin M.J. & Mortimer, A.M. 1983b A probability matrix for identification of some Streptomyces. Journal of General Microbiology 129, 1815–1830.

    CAS  Google Scholar 

  • Xu, L.H., Tiang, Y.Q., Zhang Y.F., Zhao, L.X. & Jiang, C.L. 1998 Streptomyces thermogriseus, a new species of the genus Streptomyces from soil, lake and hot-spring. International Journal of Systematic Bacteriology 48, 1089–1093.

    Article  Google Scholar 

  • Yoon, J.H., Lee, S.T. & Park, Y.H. 1998 Inter and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. International Journal of Systematic Bacteriology 48, 187–194.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Doralice Rodrigues Sacramento, Rosalie Reed Rodrigues Coelho, Márcia Dutra Wigg, Luiz Fernando de Toledo Luna Linhares, Marta Gonçalves Matos dos Santos & Luzia Teixeira de Azevedo Soares Semêdo

  2. Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Antonio Jorge Ribeiro da Silva

Authors
  1. Doralice Rodrigues Sacramento
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  2. Rosalie Reed Rodrigues Coelho
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  3. Márcia Dutra Wigg
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  4. Luiz Fernando de Toledo Luna Linhares
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  5. Marta Gonçalves Matos dos Santos
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  6. Luzia Teixeira de Azevedo Soares Semêdo
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  7. Antonio Jorge Ribeiro da Silva
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Correspondence to Rosalie Reed Rodrigues Coelho.

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Sacramento, D.R., Coelho, R.R.R., Wigg, M.D. et al. Antimicrobial and antiviral activities of an actinomycete (Streptomyces sp.) isolated from a Brazilian tropical forest soil. World Journal of Microbiology and Biotechnology 20, 225–229 (2004). https://doi.org/10.1023/B:WIBI.0000023824.20673.2f

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  • DOI: https://doi.org/10.1023/B:WIBI.0000023824.20673.2f

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