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Superoxide Dismutase Transgenes in Sugarbeets Confer Resistance to Oxidative Agents and the Fungus C. beticola

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Abstract

Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of sugarbeet petioles. The transgenic plants were subjected to treatments known to cause oxidative stress, such as the herbicide methyl viologen and a natural photosensitizer toxin produced by the fungus Cercospora beticola, namely cercosporin. The transgenic plants exhibited increased tolerance to methyl viologen, to pure cercosporin, as well as to leaf infection with the fungus C. beticola.

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Author notes

  1. Christos Vasilikiotis

    Present address: ESPM – Division of Insect Biology, University of California Berkeley, 201 Welman, Berkeley, CA, 94720-3112, USA

Authors and Affiliations

  1. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 261, Thessaloniki, 540 06, Greece

    Konstantinos Tertivanidis, Catherine Goudoula, Christos Vasilikiotis, Efthymia Hassiotou & Athanasios Tsaftaris

  2. Department of Life Sciences, Bar-Ilan University, Ramat Gan, 52900, Israel

    Rafael Perl-Treves

  3. Center of Research and Technology, Institute of Agrobiotechnology, P.O. Box 361, 570 01, Thermi, Thessaloniki, Greece

    Athanasios Tsaftaris

Authors
  1. Konstantinos Tertivanidis
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  2. Catherine Goudoula
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  3. Christos Vasilikiotis
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  4. Efthymia Hassiotou
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  5. Rafael Perl-Treves
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  6. Athanasios Tsaftaris
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Correspondence to Athanasios Tsaftaris.

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Tertivanidis, K., Goudoula, C., Vasilikiotis, C. et al. Superoxide Dismutase Transgenes in Sugarbeets Confer Resistance to Oxidative Agents and the Fungus C. beticola . Transgenic Res 13, 225–233 (2004). https://doi.org/10.1023/B:TRAG.0000034610.35724.04

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  • DOI: https://doi.org/10.1023/B:TRAG.0000034610.35724.04

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