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Engineering the Chloroplast Encoded Proteins of Chlamydomonas

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Abstract

Over a decade ago (1988), John Boynton and colleagues successfully transformed the chloroplast genome of chlamydomonas for the first time by complementation of a chloroplast deletion mutant. Since the first demonstration of chloroplast transformation the function and structure of many chloroplast encoded subunits of the photosynthetic apparatus has been characterized by site-directed mutagenesis. With the completion of the sequencing of the Chlamydomonas chloroplast genome the genetic tools are now in hand to characterize structure—function relationships for each of the chloroplast-encoded proteins of the photosynthetic apparatus.

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References

  • Allen JF (2002) Plastoquinone redox control of chloroplast thylakoid protein phosphorylation and distribution of excitation energy between photosystems: discovery, background, implications. Photosynth Res 73: 139–148

    Article  PubMed  CAS  Google Scholar 

  • Blomberg MRA, Siegbahn PEM, Styring S, Babcock GT, Aakermark B and Korall P (1997) A quantum chemical study of hydrogen abstraction from manganese-coordinated water by a tyrosyl radical: a model for water oxidation in Photosystem II.J Am Chem Soc 119: 8285–8292

    Article  CAS  Google Scholar 

  • Blubaugh DJ and Govindjee (1988) The molecular mechanism of the bicarbonate effect at the plastoquinone reductase site of photosynthesis. Photosynth Res 19: 85–128

    Article  CAS  Google Scholar 

  • Boynton JE and Gillham NW (1993) Chloroplast transformation in Chlamydomonas. Meth Enzymol 217: 510–536

    Article  PubMed  CAS  Google Scholar 

  • Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB and Sanford JC (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240: 1534–1541

    PubMed  CAS  Google Scholar 

  • Dorlet P, Xiong L, Sayre RT and Un S (2001) High-field EPR study of the pheophytin anion radical in wild type and D1-130 mutants of Photosystem II in Chlamydomonas reinhardtii. J Biol Chem 276: 22313–22316

    Article  PubMed  CAS  Google Scholar 

  • Forster B, Heifetz PB, Lardans A Boynton JE and Gillham NW (1997) Herbicide resistance and growth of D1 ALA (251) mutants in Chlamydomonas. Z Naturforsch C 52: 654–664

    Google Scholar 

  • Fromme P and Mathis P (2004) Unraveling the Photosystem I reaction center: a history, or the sum of many efforts. Photosynth Res 80: 109–124 (this issue)

    Article  PubMed  CAS  Google Scholar 

  • Goldschmidt-Clermont M (1991) Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for the site-directed transformation of Chlamydomonas. Nuc Acids Res 19: 4083–4089

    CAS  Google Scholar 

  • Govindjee and van Rensen JJS (1993) Photosystem II reaction center and the bicarbonate. In: Deisenhofer J and Norris JR (eds) The Photosynthetic Reaction Center, Vol I, pp 357–388. Academic Press, New York

    Google Scholar 

  • Guergova-Kuras M, Boudreaux B, Joliot A, Joliot P and Redding K (2001) Evidence for two active branches for electron transfer in Photosystem I. PNAS 98: 4437–4442

    Article  PubMed  CAS  Google Scholar 

  • Harris EH (1989) The Chlamydomonas Sourcebook. John Wiley, New York

    Google Scholar 

  • Heifetz PB, Lers A, Turpin DH Gillham NW, Boynton JE and Osmond CB (1997) DR and SPR/SR mutations of Chlamydomonas reinhardtii affecting D1 protein function and synthesis define two independent steps leading to chronic photoinhibition and confer differential fitness. Plant Cell Environ 20: 1145–1157

    Article  CAS  Google Scholar 

  • Hu C-Y, Houseman ALP, Morgan L, Webber AN and Frasch WD (1996) Catalytic and EPR studies of the βE204Q mutant of the chloroplast F1-ATPase from Chlamydomonas reinhardti. Biochem 35: 12201–12211

    Article  CAS  Google Scholar 

  • Hutchison RS and Sayre RT (1995) Site specific mutagenesis at histidine 118 of the Photosystem II D1 protein of Chlamydomonas reinhardtii. In: Mathis P (ed) Photosynthesis: from Light to Biosphere, Vol I, pp 471–474. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Hutchison RS, Roffey RA and Sayre RT (1996a) Chloroplast transformation. In: Andersson B, Salter AH and Barber J (eds) Molecular Genetics of Photosynthesis, Frontiers in Molecular Biology, pp 180–196. Oxford University Press, Oxford

    Google Scholar 

  • Hutchison RS, Xiong J, Sayre RT and Govindjee (1996b) Construction and characterization of a Photosystem II D1 mutant (arginine-269-glycine) of Chlamydomonas reinhardtii. Biochim Biophys Acta 1277: 83–92

    Article  PubMed  Google Scholar 

  • Johanningmeier U and Heiss S (1993) Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene. Plant Mol Biol 22: 91–99

    Article  PubMed  CAS  Google Scholar 

  • Johanningmeier U, Sopp G, Brauner M, Altenfeld U, Orawski G and Oettmeier W (2000) Herbicide resistance and supersensitivity in Ala(250) or Ala(251) mutants of the D1 protein in Chlamydomonas reinhardtii. Pesticide Biochem Physiol 66: 9–19

    Article  CAS  Google Scholar 

  • Johnston HG, Wang J, Sayre RT and Gustafson T (2000)Fluorescence decay kinetics of wild type and D2-H117N mutant Photosystem II reaction centers isolated from Chlamydomonas reinhardtii. J Phys Chem B 104: 4777–4781

    Google Scholar 

  • Kamiya N and Shen JR (2003) Crystal structure of oxygen-evolving Photosystem II from Thermosynechococcus vulcanus at 3.7-Å resolution. Nature 100: 98–103

    CAS  Google Scholar 

  • Koulougliotis D, Innes JB and Brudvig GW (1994) Location of chlorophyllZ in Photosystem II. Biochem 33: 11814–11822

    Article  CAS  Google Scholar 

  • Kramer DM, Roffey RA, Govindjee and Sayre RT (1994) The AT thermoluminescence band from Chlamydomonas reinhardtii and the effects of mutagenesis of histidine residues on the donor side of Photosystem II D1 polypeptide. Biochim Biophys Acta 1185: 228–237

    Article  CAS  Google Scholar 

  • Lardans A, Gillham NW and Boynton JE (1997) Site-directed mutations at residue 251 of the Photosystem II D1 protein of Chlamydomonas that result in a nonphotosynthetic phenotype and impair D1 synthesis and accumulation. J Biol Chem 272: 210–216

    Article  PubMed  CAS  Google Scholar 

  • Minagawa J and Crofts AR (1994) A robust protocol for sitedirected mutagenesis of the D1 protein in Chlamydomonas reinhardtii: a PCR-spliced psbA gene in a plasmid conferring spectinmycin resistance was introduced into a psbA deletion strain. Photosynth Res 42: 121–131

    Article  CAS  Google Scholar 

  • Minagawa J, Kramer DM, Kanazawa A and Crofts AR (1996) Donor-side photoinhibition in Photosystem II from Chlamydomonas reinhardtii upon mutation of tyrosine-Z in the D1 polypeptide to phenylalanine. FEBS Lett 389: 199–202

    Article  PubMed  CAS  Google Scholar 

  • Nelson N and Ben-Shem A (2002) Photosystem I reaction center: past and future. Photosynth Res 73: 193–206

    Article  PubMed  CAS  Google Scholar 

  • Przibilla E, Heiss S, Johanningmeier U and Trebst A (1991) Sitespecific mutagenesis of the D1 subunit of Photosystem II in wildtype Chlamydomonas. Plant Cell 3: 169–174

    Article  PubMed  CAS  Google Scholar 

  • Randolph-Anderson BL, Sato R, Johnson AM, Harris EH, Hauser CR, Oeda K, Ishige F, Nishio S, Gillham NW and Boynton JE (1998) Isolation and characterization of a mutant protoporphyrinogen oxidase gene from Chlamydomonas reinhardtii conferring resistance to porphyric herbicides.Plant Mol Biol 38: 839–859

    Article  PubMed  CAS  Google Scholar 

  • Redding K, Macmillan F, Leibl W, Brettel K, Hanley J, Rutherford AW, Breton J and Rochaix J-D (1998) A systematic survey of conserved histidines in the core subunits of Photosystem I by site-directed mutagenesis reveals the likely axial ligands of P700. EMBO J 17: 50–60

    Article  Google Scholar 

  • Renger G (2003) Apparatus and mechanisms of the photosynthetic oxygen evolution: historical lines of research and current state of the art. Photosynth Res 76: 269–288

    Article  PubMed  CAS  Google Scholar 

  • Rochaix J-D (2002) The three genomes of Chlamydomonas. Photosynth Res 73: 285–293

    Article  PubMed  CAS  Google Scholar 

  • Rochaix J-D, Goldschmidt-Clermont and Merchant S (eds) (1998) Molecular Biology of Chlamydomonas: Chloroplasts and Mitochondria. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Roffey R and Sayre RT (1990) Isolation of site-specific chloroplast psbA mutants having null phenotypes by co-transformation.Plant Physiol 93: 22–22

    Google Scholar 

  • Roffey RA, Golbeck JH, Hille CR and Sayre RT (1991) Photosynthetic electron transport in genetically altered Photosystem II reaction centers of chloroplasts. Proc Natl Acad Sci USA 88: 9122–9126

    Article  PubMed  CAS  Google Scholar 

  • Roffey RA, Kramer DM, Govindjee and Sayre RT (1994a) Lumenal side histidine mutations in the D1 protein of Photosystem II affect donor side electron transfer in Chlamydomonas reinhardtii. Biochim Biophys Acta 1185: 257–270

    Article  PubMed  CAS  Google Scholar 

  • Roffey RA, van Wijk K, Sayre RT and Styring S (1994b) Spectroscopic characterization of Tyrosine-Z in Histidine 190 mutants of the D1 protein in Photosystem II (PS II) in Chlamydomonas reinhardtii. J Biol Chem 269: 5115–5121

    PubMed  CAS  Google Scholar 

  • Ruffle SV and Sayre RT (1998) Functional Analysis of Photosystem IIIn: Molecular Biology of Chlamydomonas: Chloroplasts and Mitochondria, pp 287–322. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Ruffle SV, Wang J, Johnston H, Gustafson TG, Hutchison R, Minagawa J, Crofts A and Sayre RT (2001) Photosystem II peripheral accessory chlorophyll mutants in Chlamydomonas re inhardtii; biochemical characterization and sensitivity to photoinhibition. Plant Physiol 127: 633–644

    Article  PubMed  CAS  Google Scholar 

  • Satoh Ki (2003) The identification of the Photosystem II reaction center. Photosynth Res 76: 233–240

    Article  PubMed  CAS  Google Scholar 

  • Schelvis J, van Noort P, Aartsma T and van Gorkom H (1994) Energy transfer, charge separation and pigment arrangement in the reaction center of Photosystem II. Biochim Biophys Acta 1184: 242–250

    Article  CAS  Google Scholar 

  • Seibert M and Wasielewski M (2003) The isolated Photosystem II reaction center — first attempts to directly measure the kinetics of primary charge separation. Photosynth Res 76: 263–268.

    Article  PubMed  CAS  Google Scholar 

  • Stewart DH, Cua A, Chisholm DA, Diner BA, Bocian DF and Brudvig GW (1998) Identification of histidine 118 in the D1 polypeptide of Photosystem II as the axial ligand to chlorophyll Z. Biochem 37: 10040–10046

    Article  CAS  Google Scholar 

  • Svensson B, Vass I, Cedergren E and Styring S (1990) Structure of donor side components in Photosystem II predicted by computer modeling. EMBO J 9: 2051–2059

    Google Scholar 

  • Thompson LK and Brudvig GW (1988) Cytochrome b-559 may function to protect Photosystem II from photoinhibition. Biochemistry 27: 6653–6658

    Article  PubMed  CAS  Google Scholar 

  • Vass I (2003) The history of photosynthetic thermoluminescence. Photosynth Res 76: 303–318

    Article  PubMed  CAS  Google Scholar 

  • Wang J, Gosztola D, Ruffle SV, Hemann C, Seibert M, Wasielewski MR, Hille R, Gustafson TL and Sayre RT (2002) Functional asymmetry of Photosystem II D1 and D2 peripheral chlorophyll mutants of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 99: 4091–4096

    Article  PubMed  CAS  Google Scholar 

  • Webber AN, Su H, Bingham SE, Kaess H, Krabben L, Kuhn M, Jordan R, Schlodder E and Lubitz W (1996) Site-directed mutations affecting the spectroscopic characteristics and midpoint potential of the primary donor in Photosystem I. Biochemistry 35: 12857–12863

    Article  PubMed  CAS  Google Scholar 

  • Witt HT (2004) Steps on the way to building blocks, topologies, crystals and X-ray structural analysis of Photosystems I and II of water-oxidizing photosynthesis. Photosynth Res 80: 85–107 (this issue)

    Article  CAS  Google Scholar 

  • Wurtz EA, Boynton JE and Gillham NW (1977) Perturbation of chloroplast DNA amounts and chloroplast gene transmission in Chlamydomonas reinhardtii by 5-fluorodeoxyuridine. Proc Natl Acad Sci USA 74: 4552–4556

    Article  PubMed  CAS  Google Scholar 

  • Xiong J, Subramaniam S and Govindjee (1996) Modeling of the D1/D2 proteins and cofactors of the Photosystem II reaction center: implications for herbicide and bicarbonate binding. Prot Sci 5: 2054–2073

    Article  CAS  Google Scholar 

  • Xiong J, Subramaniam S and Govindjee (1998a) A knowledgebased three dimensional model of the Photosystem II reaction center of Chlamydomonas reinhardtii. Photosynth Res 56: 229–254

    Article  CAS  Google Scholar 

  • Xiong J, Minagawa J, Crofts AR and Govindjee (1998b) Loss of inhibition by formate in newly constructed Photosystem II D1 mutants, D1-R257E and D1-R257M, of Chlamydomonas reinhardtii. Biochim Biophys Acta 1365: 473–491

    Article  PubMed  CAS  Google Scholar 

  • Zito F, Finazzi G, Delsome R, Nitschke W, Picot D and Wollman F-A (1999) The Q0 site of cytochrome b 6 f complexes controls the activation of the LHC kinase. EMBO J 18: 2961–2969

    Article  Google Scholar 

  • Zouni A, Witt H-T, Kern J, Fromme P, Krauss N, Saenger W and Orth P (2001) Crystal structure of Photosystem II from Synechococcus elongatus at 3.8Å resolution. Nature 409: 739–743

    Article  PubMed  CAS  Google Scholar 

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  1. Department of Plant Biology, Ohio State University, Columbus, OH, 43210, USA

    Ling Xiong & Richard T. Sayre

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  1. Ling Xiong
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  2. Richard T. Sayre
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Xiong, L., Sayre, R.T. Engineering the Chloroplast Encoded Proteins of Chlamydomonas. Photosynthesis Research 80, 411–419 (2004). https://doi.org/10.1023/B:PRES.0000030458.98624.37

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