Abstract
A hydroponic experiment was carried out to study genotypic differences in effect of Al and Cd on growth and antioxidant enzyme activities by using 2 two-row winter barley genotypes (Hordeum vulgare L.) with different Al resistance, the relatively resistant Gebeina and the sensitive Shang 70–119. The seedling growth, presented as shoot height, root length and dry weight of root and shoot, and tillers per plant were inhibited by all stress treatments, including low pH, 100 μM Al (pH 4.0) and 1.0 μM Cd+100 μM Al (pH 4.0), while 1.0 μM Cd showed a slight stimulation of growth. The inhibition was more severe in 1.0 μM Cd +100 μM Al (pH 4.0) than in 100 μM Al (pH 4.0), indicating that the effect of Cd and Al is synergistic. Al-sensitive genotype Shang 70–119 was more inhibited than Al-resistant genotype Gebeina. Proline concentration in leaves was significantly increased when plants were exposed to all stress treatments, being more pronounced in Shang 70–119 than in Gebeina. A highly significant increase in malonaldehyde (MDA) concentration, and a stimulation of superoxide dismutase (SOD) and peroxidase (POD) activities were recorded in the plants subjected to low pH, 100 μM Al (pH 4.0) and 1.0 μM Cd +100 μM Al(pH 4.0) treatments, and the extent of the increase varied greatly depending on concentration and time of exposure. Shang 70–119 had a higher MDA concentration, and less increase in SOD activity when first exposed than Gebeina had.
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References
Alia K V, Prasad S K and Pardha S P 1995 Effect of zinc on free radical and proline in Brassica juncea and Cajanus cajan. Phytochemistry 39, 45–47.
Asada K 1994 Production and action of active oxygen species in photosynthetic tissues. In Causes of photooxidative stress and Amelioration of defense systems in Plants. Eds. C H Foyer and P M Mullineaux. pp. 77–104. CRC Press, Boca Raton, FL.
Basu U, Godbold D and Tayor G J 1994 Aluminum resistance in Triticum aestivum associated with enhanced exudation of malate. Plant Physiol. 144, 747–753.
Boscolo P R S, Menossi M and Jorgea R A 2003 Aluminum-induced oxidative stress in maize. Phytochemistry 62, 181–189.
Bowler C, Van Montagu M and Inze D 1992 Superoxide dismutase and stress tolerence. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 83–116.
Cakmak K B and Horst W J 1991 Effect of aluminum on lipid peroxidation, Superoxide dismutase, catalase and peroxidase activities in rot tips of soybean (Glycine max). Physiol. Plant. 83, 463–468.
Chaoui A, Mazhoudi S, Ghorbal M H and Ferjani E E L 1997 Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus ulgaris L.). Plant Sci. 127, 139–147.
Delhaize E and Ryan P R 1995 Aluminum toxicity and tolerance in plants. Plant Physiol. 107, 315–321.
Delhaize E, Ryan P R and Randall P J 1993 Aluminum tolerance in wheat (Triticum aestivum L.) II. Aluminum-stimulated excretion of malic acid from root apices. Plant Physiol. 103, 695–702.
Foursy A and Fares K 2002 Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ. Exp. Bot. 47, 39–50.
Foyer C, Lelandais M and Kunert J J 1994 Photooxidative stress in plants. Physiol. Plant, 92, 696–717.
Galvez L and Clark R B 1991 Nitrate and amnonium uptake and solution pH changes for Al-sensitive sorghum (Sorghum bicolor) genotypes grown with and without aluminum. In Eds. R J Wright et al. pp. 805–814. Plant-soil Interaction at Low pH. Kluwer Academic Publishers, Dordrecht.
Gille G and Sigler K 1995 Oxidative stress and living cells. Folia Microbiol. 40, 131–152.
Greger M L, Kautskey T and Sandberg A 1995 A tentative model of Cd uptake in Otamogeton petinatus in relation to salinity. Environ. Exp. Bot. 35, 215–225.
Guo T R, Zhang G P, Lu W Y, Wu H P and Zhou M X 2003 Effect of Al on dry matter accumulation and Al and nutrients in barleys differing in Al tolerance, Plant Nutri. Fert. Sci. in press.
Ikegawa H, Yamamoto Y and Matsumoto H, 2000 Responses to aluminum of suspension-cultured tobacco cells in a simple calcium solution. Soil Sci. Plant Nutri. 46, 503–514.
Kasai M, Sasaki M, Yamamoto Y and Matsumoto H 1992 Al increases K+ efflux and activities of ATP-dependent and PPi-dependent Ht pumps of tonoplast-enriched vesicles from barley roots. Plant Cell Physiol. 33, 1035–1039.
Keltjens W G and Ulden P S R 1987 Effect of Al on nitrogen (NH4 and NO3) uptake, nitrate reductase activity and proton releaser in two sorghum cultivars different in Al tolerance. Plant Soil, 104, 227–234.
Kochian L V 1995 Cellular mechanisms of aluminum toxicity and resistance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46, 237–260.
Lin C C and Kao C H 2000 Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regul. 30, 151–155.
Pellet D M, Grunes D L and Kochian L V 1995 Organic acid exudation as an aluminum-tolerance mechanism in maize (Zea mays L). Planta, 196, 788–795.
Richards I R, Clayton C J and Reeve A J 1998 Effect of long-term fertilizer phosphorus application on soil and crop phosphorus and cadmium content. J. Agri. Sci. Cambridge, 131, 187–195.
Shahandeh H and Hossner L R 2002 Plant screening for chromium phytoremediation. Internl. J. Phytoremed. 2, 31–51.
Taylor G J, Basu A, Basu U and Slaski J J 1997 Al-induced 51-kilodalton membrane-bound proteins are associated with resistance to Al in a segregating population of wheat. Plant Physiol. 114, 363–372.
Wagatsuma T and Yamasaka K 1985 Relationship between differential aluminum tolerance and plant-induced pH change of medium among barley cultivars. Soil Sci. Plant Nutri. 31, 521–535.
Wu F B and Zhang G P 2002 Genotypic Variation in Kernel Heavy Metal Concentrations in Barley and as Affected by Soil Factors. J. Plant Nutri. 25, 1163–1173.
Yamamoto Y, Hachiya A and Matsumoto H, 1997 Oxidative damage to membrane by a combination of aluminum and iron in suspension-cultured tobacco cells. Plant Cell Physiol. 38, 1333–1339.
Yamamoto, Y, Kobayashi Y and Matsumoto H, 2001 Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol. 125, 199–208.
Zhang J and Kirkham M B 1994 Drought-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol. 35, 489–495.
Zhang X Z 1992 The measurement and mechanism of lipid peroxidation and SOD, POD and CAT activities in biological system. In Research Methodology of Crop Physiology. Ed. X Z Zhang (in Chinese). pp. 208–211. Agriculture Press, Beijing.
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Guo, T., Zhang, G., Zhou, M. et al. Effects of aluminum and cadmium toxicity on growth and antioxidant enzyme activities of two barley genotypes with different Al resistance. Plant and Soil 258, 241–248 (2004). https://doi.org/10.1023/B:PLSO.0000016554.87519.d6
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DOI: https://doi.org/10.1023/B:PLSO.0000016554.87519.d6